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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Proximity ligation assay [1]
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- Product number
- H00003172-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003172-D01P, RRID:AB_1673832
- Product name
- HNF4A purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human HNF4A protein.
- Antigen sequence
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLT
MGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGK
HYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVD
KDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTR
RSSYEDSSLPSINALLQAEVLSRQITSPVSGINGD
IRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCE
LPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLL
GNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQ
IDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQV
QVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQ
MIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHA
HHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMSTP
ETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQ
PTITKQEVI- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Transcriptional regulation of PCFT by KLF4, HNF4α, CDX2 and C/EBPα: implication in its site-specific expression in the small intestine.
Furumiya M, Inoue K, Ohta K, Hayashi Y, Yuasa H
Biochemical and biophysical research communications 2013 Feb 8;431(2):158-63
Biochemical and biophysical research communications 2013 Feb 8;431(2):158-63
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HNF4A expression in transfected 293T cell line (H00003172-T01) by HNF4A MaxPab polyclonal antibody.Lane 1: HNF4A transfected lysate(51.60 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HNF4A MaxPab rabbit polyclonal antibody. Western Blot analysis of HNF4A expression in Raw 264.7.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between HNF4A and CTNNB1. HeLa cells were stained with anti-HNF4A rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
