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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 66785-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LACTB Monoclonal Antibody (3C3C3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: DPEASPLAE PPQEQSLAPW SPQTPAPPCS RCFARAIESS RDLLHRIKDE VGAPGIVVGV SVDGKEVWSE GLGYADVENR VPCKPETVMR IASISKSLTM VALAKLWEAG KLDLDIPVQH YVPEFPEKEY EGEKVSVTTR LLISHLSGIR HYEKDIKKVK EEKAYKALKM MKENVAFEQE KEGKSNEKND FTKFKTEQEN EAKCRNSKPG KKKNDFEQGE LYLREKFENS IESLRLFKND PLFFKPGSQF LYSTFGYTLL AAIVERASGC KYLDYMQKIF HDLDMLTTVQ EENEPVIYNR AR (73-373 aa encoded by BC067288)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3C3C3
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66785-1-IG (LACTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66785-1-Ig (LACTB antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
