Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21998 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMPK gamma-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HepG2.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of AMPK gamma-2 using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an AMPK gamma-2 polyclonal antibody (Product # PA5-21998) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AMPK gamma-2 using 30 µg of HeLa S3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an AMPK gamma-2 polyclonal antibody (Product # PA5-21998) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AMPK gamma-2 was performed by separating 30 µg of whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AMPK gamma-2 Polyclonal Antibody (Product # PA5-21998) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AMPK gamma-2 in paraformaldehyde-fixed HeLa cells using an AMPK gamma-2 polyclonal antibody (Product # PA5-21998) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AMPK gamma-2 antibody (Product # PA5-21998) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AMPK gamma-2 Polyclonal Antibody detects PRKAG2 protein at cytosol on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer tissue. AMPK gamma-2 Polyclonal Antibody (Product # PA5-21998) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.