H00005756-M05

antibody from Abnova Corporation

Targeting: TWF1 A6, PTK9

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005756-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005756-M05, RRID:AB_534997

Product name

PTK9 monoclonal antibody (M05), clone 1D12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PTK9.

Antigen sequence

VAFPISREAFQALEKLNNRQLNYVQLEIDIKNEII
ILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEG
DYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEI
VERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQH
AHKQSSAKPKGPAGKRGIRRLIRGPAETEATTD


Isotype

IgG

Antibody clone number

1D12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PTK9 expression in transfected 293T cell line by PTK9 monoclonal antibody (M05), clone 1D12.Lane 1: PTK9 transfected lysate(43.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PTK9 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to TWF1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol