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- Antibody Data
- Antigen structure
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- Western blot [2]
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- Immunohistochemistry [1]
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- Product number
- PA5-29312 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTK9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, NIH-3T3.
- Concentration
- 1 mg/mL
Submitted references Novel plant microRNAs from broccoletti sprouts do not show cross-kingdom regulation of pancreatic cancer.
Xiao X, Sticht C, Yin L, Liu L, Karakhanova S, Yin Y, Georgikou C, Gladkich J, Gross W, Gretz N, Herr I
Oncotarget 2020 Apr 7;11(14):1203-1217
Oncotarget 2020 Apr 7;11(14):1203-1217
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PTK9 Polyclonal Antibody (Product # PA5-29312). Sample (30 µg of whole cell lysate). Lane A: H1299. 10% SDS PAGE. PTK9 Polyclonal Antibody (Product # PA5-29312) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PTK9 Polyclonal Antibody (Product # PA5-29312). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. PTK9 Polyclonal Antibody (Product # PA5-29312) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PTK9 in paraformaldehyde-fixed HeLa cells using a PTK9 polyclonal antibody (Product # PA5-29312) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PTK9 Polyclonal Antibody detects PTK9 protein at cytoplasm on SW480 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SW480 xenograft. PTK9 Polyclonal Antibody (Product # PA5-29312) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Lipofection of broccoletti-miRs in PDA cells does not induce the expression of target genes. ( A ) BxPc-3 and Bx-Gem cells were transfected with bra-miR156g-5p, Myseq-330, miR-D (50 nM each), or a miR-NG oligonucleotide (50 nM), which served as a mock control. At 24 h after transfection, the RNA was isolated, followed by reverse transcription. The samples were validated by TaqMan (r) miR assay. RNU44 was used to normalize the expression level, and the average fold change of the mock control was set to 1. The relative fold changes x1000 are presented. Experiments were performed in triplicate, and the data are shown as the means +- SD ( ** P < 0.01). ( B ) Proteins were harvested from BxPc-3 cells at 24 h after lipofection, and western blot analysis was performed for p53, XIAP, FoxO1, c-Myc, Akt1 and caspase-3. beta-actin served as a control for equal loading conditions. The protein sizes are shown in kilodaltons (kD). The band intensities were measured using ImageJ and are shown below the bands. The band intensities were normalized to beta-actin. ( C ) Total proteins were harvested from BxPc-3 cells at 24 h after lipofection, and PTK9 protein levels were evaluated via western blot analysis and examined as described above.
