Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG54128 - Provider product page
- Provider
- Arigo
- Product name
- anti-AMPK beta 1 antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1A7-E11-E9
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: AMPK beta 1 in 3T3, HeLa, PC-12, COS7 and MDA-MB-468 cell lysates stained with AMPK beta 1 antibody [1A7-E11-E9] (ARG54128) (diluted in 1: 1000). Predicted band size: 38KDa. Observed band size: 38KDa. Exposure time: 5min.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed with 1% Paraformaldehyde and stained with AMPK beta 1 antibody [1A7-E11-E9] (ARG54128) (diluted in 1:100).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: HeLa cell lysates immunoprecipitated and stained by AMPK beta 1 antibody [1A7-E11-E9] (ARG54128).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Breast cancer stained with AMPK beta 1 antibody [1A7-E11-E9] (ARG54128) (diluted in 1/200). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6. 0).