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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-8992 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8992, RRID:AB_2155784
- Product name
- Anti-NR3C1
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Short-term recognition memory impairment is associated with decreased expression of FK506 binding protein 51 in the aged mouse brain.
Time-resolved expression profiling of the nuclear receptor superfamily in human adipogenesis.
Thymic changes after chorioamnionitis induced by intraamniotic lipopolysaccharide in fetal sheep.
Glucocorticoid receptor knock down reveals a similar apoptotic threshold but differing gene regulation patterns in T-cell and pre-B-cell acute lymphoblastic leukemia.
Regulation of pyruvate dehydrogenase kinase isoform 4 (PDK4) gene expression by glucocorticoids and insulin.
Nestin modulates glucocorticoid receptor function by cytoplasmic anchoring.
Molecular characterization of SMILE as a novel corepressor of nuclear receptors.
Protopanaxadiol and protopanaxatriol bind to glucocorticoid and oestrogen receptors in endothelial cells.
Molecular regulation of the hypothalamic-pituitary-adrenal axis in adult male guinea pigs after prenatal stress at different stages of gestation.
Glucocorticoid receptor-mediated expression of caldesmon regulates cell migration via the reorganization of the actin cytoskeleton.
Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells.
A conserved molecular mechanism is responsible for the auto-up-regulation of glucocorticoid receptor gene promoters.
Differential effects of hydrocortisone and TNFalpha on tight junction proteins in an in vitro model of the human blood-brain barrier.
Transcription factors in muscle atrophy caused by blocked neuromuscular transmission and muscle unloading in rats.
Glucocorticoid receptor-JNK interaction mediates inhibition of the JNK pathway by glucocorticoids.
Soontornniyomkij V, Risbrough VB, Young JW, Wallace CK, Soontornniyomkij B, Jeste DV, Achim CL
Age (Dordrecht, Netherlands) 2010 Sep;32(3):309-22
Age (Dordrecht, Netherlands) 2010 Sep;32(3):309-22
Time-resolved expression profiling of the nuclear receptor superfamily in human adipogenesis.
Lahnalampi M, Heinäniemi M, Sinkkonen L, Wabitsch M, Carlberg C
PloS one 2010 Sep 27;5(9):e12991
PloS one 2010 Sep 27;5(9):e12991
Thymic changes after chorioamnionitis induced by intraamniotic lipopolysaccharide in fetal sheep.
Kunzmann S, Glogger K, Been JV, Kallapur SG, Nitsos I, Moss TJ, Speer CP, Newnham JP, Jobe AH, Kramer BW
American journal of obstetrics and gynecology 2010 May;202(5):476.e1-9
American journal of obstetrics and gynecology 2010 May;202(5):476.e1-9
Glucocorticoid receptor knock down reveals a similar apoptotic threshold but differing gene regulation patterns in T-cell and pre-B-cell acute lymphoblastic leukemia.
Schwartz JR, Sarvaiya PJ, Vedeckis WV
Molecular and cellular endocrinology 2010 May 14;320(1-2):76-86
Molecular and cellular endocrinology 2010 May 14;320(1-2):76-86
Regulation of pyruvate dehydrogenase kinase isoform 4 (PDK4) gene expression by glucocorticoids and insulin.
Connaughton S, Chowdhury F, Attia RR, Song S, Zhang Y, Elam MB, Cook GA, Park EA
Molecular and cellular endocrinology 2010 Feb 5;315(1-2):159-67
Molecular and cellular endocrinology 2010 Feb 5;315(1-2):159-67
Nestin modulates glucocorticoid receptor function by cytoplasmic anchoring.
Reimer R, Helmbold H, Szalay B, Hagel C, Hohenberg H, Deppert W, Bohn W
PloS one 2009 Jun 29;4(6):e6084
PloS one 2009 Jun 29;4(6):e6084
Molecular characterization of SMILE as a novel corepressor of nuclear receptors.
Xie YB, Nedumaran B, Choi HS
Nucleic acids research 2009 Jul;37(12):4100-15
Nucleic acids research 2009 Jul;37(12):4100-15
Protopanaxadiol and protopanaxatriol bind to glucocorticoid and oestrogen receptors in endothelial cells.
Leung KW, Leung FP, Mak NK, Tombran-Tink J, Huang Y, Wong RN
British journal of pharmacology 2009 Feb;156(4):626-37
British journal of pharmacology 2009 Feb;156(4):626-37
Molecular regulation of the hypothalamic-pituitary-adrenal axis in adult male guinea pigs after prenatal stress at different stages of gestation.
Kapoor A, Leen J, Matthews SG
The Journal of physiology 2008 Sep 1;586(17):4317-26
The Journal of physiology 2008 Sep 1;586(17):4317-26
Glucocorticoid receptor-mediated expression of caldesmon regulates cell migration via the reorganization of the actin cytoskeleton.
Mayanagi T, Morita T, Hayashi K, Fukumoto K, Sobue K
The Journal of biological chemistry 2008 Nov 7;283(45):31183-96
The Journal of biological chemistry 2008 Nov 7;283(45):31183-96
Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells.
Arenas F, Hervias I, Uriz M, Joplin R, Prieto J, Medina JF
The Journal of clinical investigation 2008 Feb;118(2):695-709
The Journal of clinical investigation 2008 Feb;118(2):695-709
A conserved molecular mechanism is responsible for the auto-up-regulation of glucocorticoid receptor gene promoters.
Geng CD, Schwartz JR, Vedeckis WV
Molecular endocrinology (Baltimore, Md.) 2008 Dec;22(12):2624-42
Molecular endocrinology (Baltimore, Md.) 2008 Dec;22(12):2624-42
Differential effects of hydrocortisone and TNFalpha on tight junction proteins in an in vitro model of the human blood-brain barrier.
Förster C, Burek M, Romero IA, Weksler B, Couraud PO, Drenckhahn D
The Journal of physiology 2008 Apr 1;586(7):1937-49
The Journal of physiology 2008 Apr 1;586(7):1937-49
Transcription factors in muscle atrophy caused by blocked neuromuscular transmission and muscle unloading in rats.
Nordquist J, Höglund AS, Norman H, Tang X, Dworkin B, Larsson L
Molecular medicine (Cambridge, Mass.) 2007 Sep-Oct;13(9-10):461-70
Molecular medicine (Cambridge, Mass.) 2007 Sep-Oct;13(9-10):461-70
Glucocorticoid receptor-JNK interaction mediates inhibition of the JNK pathway by glucocorticoids.
Bruna A, Nicolàs M, Muñoz A, Kyriakis JM, Caelles C
The EMBO journal 2003 Nov 17;22(22):6035-44
The EMBO journal 2003 Nov 17;22(22):6035-44
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 86
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
