GTX60360

antibody from GeneTex

Targeting: NR3C1 GR, GRL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Gel shift (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX60360

Provider

GeneTex

Product name

Glucocorticoid receptor antibody - ChIP grade

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Storage

Store at -20°C; for long storage, store at -80¢XC. Avoid multiple freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of extracts from (A) HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), Raji and Molt cells and from (B) HeLa cells using Glucocorticoid receptor antibody.

ELISA

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sandwich ELISA was assessed using Glucocorticoid receptor antibody against recombinant hGR at a concentration of 0.5 £gg/£gl.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 £gl IP reaction solution) using 5 £gg of Glucocorticoid receptor antibody. The immunoprecipitated proteins were analysed by WB with Glucocorticoid receptor antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC analysis of rat CA1 neurons of hippocampus using Glucocorticoid receptor antibody at a concentration of 2.5 £gg/ml.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC analysis of COS-7 cells transiently overexpressing hGR using Glucocorticoid receptor antibody at a concentration of 2.5 £gg/ml.