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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- MAB0119 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0119, RRID:AB_1016991
- Product name
- NR3C1 monoclonal antibody, clone m2F8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinantt NR3C1.
- Isotype
- IgG
- Antibody clone number
- m2F8
- Storage
- Store at -20°C. For long term storage, store at -80°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Heat-induced degradation of overexpressed glucocorticoid receptor Separate protective roles of hsp90 and hsp70.
Gender-dependent alterations in corticosteroid receptor status and spatial performance following 21 days of restraint stress.
Overexpressed glucocorticoid receptor negatively regulates gene expression under conditions that favour accumulation of non-hormone-binding forms of the receptor.
Siriani D, Mitsiou DJ, Alexis MN
The Journal of steroid biochemistry and molecular biology 2005 Feb;94(1-3):93-101
The Journal of steroid biochemistry and molecular biology 2005 Feb;94(1-3):93-101
Gender-dependent alterations in corticosteroid receptor status and spatial performance following 21 days of restraint stress.
Kitraki E, Kremmyda O, Youlatos D, Alexis MN, Kittas C
Neuroscience 2004;125(1):47-55
Neuroscience 2004;125(1):47-55
Overexpressed glucocorticoid receptor negatively regulates gene expression under conditions that favour accumulation of non-hormone-binding forms of the receptor.
Siriani D, Mitsiou DJ, Alexis MN
The Journal of steroid biochemistry and molecular biology 2003 Feb;84(2-3):171-80
The Journal of steroid biochemistry and molecular biology 2003 Feb;84(2-3):171-80
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Analysis by Western blot of cell extracts using the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119).A) Analysis by Western blot of extracts from HeLa cells containing the indicated amounts of Nr3C1 (from 30 to 240 fmol) and from 5x 106 Raji cells and 5x 106 Molt cells using the MAb directed against human NR3C1.B) 300,000 HeLa cells were also analyzed by Western blot using the MAb anti-NR3C1. The monoclonal antibody directed against NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119) is used at 1 ug/mL .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Sandwich ELISA of NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119). A) Schematic representation of the sandwich ELISA, the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119) is used at 0.5 ug/mL .B) As increasing amounts of recombinant NR3C1 are used in the ELISA, the ELISA signal is proportionally increasing.
- Validation comment
- Sandwich ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry using the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119). COS-7 cells transiently overexpressing human NR3C1 were labeled with the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119) at 2.5 ug/mL followed by biotinylated second antibody and peroxidase-labeled avidin.
- Validation comment
- Immunocytochemistry
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation using the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119). The immunoprecipitation (IP) of the glucocorticoid receptor from HeLa cell extracts is performed using the monoclonal antibody directed against NR3C1 as follows : 5 ug of antibody per 5 million HeLa cells in 100 uL IP reaction solution. The IP is followed by Western blot analysis with the same antibody.
- Validation comment
- Immunoprecipitation
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry using the NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119) at 2.5 ug/mL . Here we show NR3C1 immunoreactivity in rat CA1 neurons of hippocampus.
- Validation comment
- Immunohistochemistry
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ChIP results obtained with the antibody directed against NR3C1 monoclonal antibody, clone m2F8 (Cat # MAB0119). ChIP assays were performed using HeLa cells.
- Validation comment
- ChIP
