24050-1-AP

antibody from Proteintech Group

Targeting: NR3C1 GR, GRL

Provider product page for 24050-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [52]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 24050-1-AP - Provider product page
Provider: Proteintech Group
Product name: Glucocorticoid receptor antibody
Antibody type: Polyclonal
Description: KD/KO validated Glucocorticoid receptor antibody (Cat. #24050-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NR3C1 protein structure - 24050-1-AP shown in red.

Submitted references Network Pharmacology-Based Approach to Investigate the Molecular Targets of Sinomenine for Treating Breast Cancer.

Li XM, Li MT, Jiang N, Si YC, Zhu MM, Wu QY, Shi DC, Shi H, Luo Q, Yu B
Cancer management and research 2021;13:1189-1204

Hair growth predicts a depression-like phenotype in rats as a mirror of stress traceability.

Ren SY, Zhang YN, Wang MJ, Wen BR, Xia CY, Li X, Wang HQ, Zhang RP, Zhang Y, Wang ZZ, Chen NH
Neurochemistry international 2021 Sep;148:105110

Discovery of a covalent inhibitor of heat shock protein 90 with antitumor activity that blocks the co-chaperone binding via C-terminal modification.

Li L, Chen N, Xia D, Xu S, Dai W, Tong Y, Wang L, Jiang Z, You Q, Xu X
Cell chemical biology 2021 Oct 21;28(10):1446-1459.e6

ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.

Park J, Lee J, Kim JH, Lee J, Park H, Lim C
Nucleic acids research 2021 Nov 8;49(19):11294-11311

Differential Effects of Fkbp4 and Fkbp5 on Regulation of the Proopiomelanocortin Gene in Murine AtT-20 Corticotroph Cells.

Kageyama K, Iwasaki Y, Watanuki Y, Niioka K, Daimon M
International journal of molecular sciences 2021 May 27;22(11)

Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats.

Verhaeghe R, Gao V, Morley-Fletcher S, Bouwalerh H, Van Camp G, Cisani F, Nicoletti F, Maccari S
GeroScience 2021 May 13;

Oxidative stress in retrotrapezoid nucleus/parafacial respiratory group and impairment of central chemoreception in rat offspring exposed to maternal cigarette smoke.

Lei F, Wang W, Fu Y, Wang J, Zheng Y
Reproductive toxicology (Elmsford, N.Y.) 2021 Mar;100:35-41

Honokiol Prevents Non-Alcoholic Steatohepatitis-Induced Liver Cancer via EGFR Degradation through the Glucocorticoid Receptor-MIG6 Axis.

Okuda K, Umemura A, Umemura S, Kataoka S, Taketani H, Seko Y, Nishikawa T, Yamaguchi K, Moriguchi M, Kanbara Y, Arbiser JL, Shima T, Okanoue T, Karin M, Itoh Y
Cancers 2021 Mar 25;13(7)

SRC-1 Deficiency Increases Susceptibility of Mice to Depressive-Like Behavior After Exposure to CUMS.

Wu Q, Wang B, Ntim M, Zhang X, Na XY, Yuan YH, Wu XF, Yang JY, Li S
Neurochemical research 2021 Jul;46(7):1830-1843

The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice.

Häusl AS, Brix LM, Hartmann J, Pöhlmann ML, Lopez JP, Menegaz D, Brivio E, Engelhardt C, Roeh S, Bajaj T, Rudolph L, Stoffel R, Hafner K, Goss HM, Reul JMHM, Deussing JM, Eder M, Ressler KJ, Gassen NC, Chen A, Schmidt MV
Molecular psychiatry 2021 Jul;26(7):3060-3076

Chronic glucocorticoid treatment induces hepatic lipid accumulation and hyperinsulinaemia in part through actions on AgRP neurons.

Harno E, Sefton C, Wray JR, Allen TJ, Davies A, Coll AP, White A
Scientific reports 2021 Jul 2;11(1):13776

The glucocorticoid receptor recruits the COMPASS complex to regulate inflammatory transcription at macrophage enhancers.

Greulich F, Wierer M, Mechtidou A, Gonzalez-Garcia O, Uhlenhaut NH
Cell reports 2021 Feb 9;34(6):108742

Sensitivity to behavioral stress impacts disease pathogenesis in dystrophin-deficient mice.

Lindsay A, Trewin AJ, Sadler KJ, Laird C, Della Gatta PA, Russell AP
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Dec;35(12):e22034

Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance.

Deng Y, Xia X, Zhao Y, Zhao Z, Martinez C, Yin W, Yao J, Hang Q, Wu W, Zhang J, Yu Y, Xia W, Yao F, Zhao D, Sun Y, Ying H, Hung MC, Ma L
Nature communications 2021 Dec 6;12(1):7041

Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors.

Mifsud KR, Kennedy CLM, Salatino S, Sharma E, Price EM, Haque SN, Gialeli A, Goss HM, Panchenko PE, Broxholme J, Engledow S, Lockstone H, Cordero Llana O, Reul JMHM
Nature communications 2021 Aug 6;12(1):4737

Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids.

Hu W, Jiang C, Kim M, Yang W, Zhu K, Guan D, Lv W, Xiao Y, Wilson JR, Rader DJ, Pui CH, Relling MV, Lazar MA
Cell metabolism 2021 Aug 3;33(8):1592-1609.e7

Abnormal Glu/mGluR_2/3/PI3K pathway in the hippocampal neurovascular unit leads to diabetes-related depression.

Liu J, Han YS, Liu L, Tang L, Yang H, Meng P, Zhao HQ, Wang YH
Neural regeneration research 2021 Apr;16(4):727-733

LRRK2 Inhibition Ameliorates Dexamethasone-Induced Glucose Intolerance via Prevents Impairment in GLUT4 Membrane Translocation in Adipocytes.

Imai M, Kawakami F, Kubo M, Kanzaki M, Maruyama H, Kawashima R, Maekawa T, Kurosaki Y, Kojima F, Ichikawa T
Biological & pharmaceutical bulletin 2020;43(11):1660-1668

Anti-inflammatory functions of the glucocorticoid receptor require DNA binding.

Escoter-Torres L, Greulich F, Quagliarini F, Wierer M, Uhlenhaut NH
Nucleic acids research 2020 Sep 4;48(15):8393-8407

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.

Wang L, Cheng CM, Qin J, Xu M, Kao CY, Shi J, You E, Gong W, Rosa LP, Chase P, Scampavia L, Madoux F, Spicer T, Hodder P, Xu HE, Tsai SY, Tsai MJ
Science advances 2020 May;6(18):eaaz8031

Maternal betaine protects rat offspring from glucocorticoid-induced activation of lipolytic genes in adipose tissue through modification of DNA methylation.

Zhao N, Yang S, Sun B, Feng Y, Zhao R
European journal of nutrition 2020 Jun;59(4):1707-1716

Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age.

Li H, Liang M, Wang Z, Zhang Y, Wu Q, Yang L
Journal of medicinal food 2020 Jun;23(6):565-574

Glucocorticoids rapidly inhibit cell migration through a novel, non-transcriptional HDAC6 pathway.

Kershaw S, Morgan DJ, Boyd J, Spiller DG, Kitchen G, Zindy E, Iqbal M, Rattray M, Sanderson CM, Brass A, Jorgensen C, Hussell T, Matthews LC, Ray DW
Journal of cell science 2020 Jun 11;133(11)

MicroRNA 322 Aggravates Dexamethasone-Induced Muscle Atrophy by Targeting IGF1R and INSR.

Geng H, Song Q, Cheng Y, Li H, Yang R, Liu S, Hao L
International journal of molecular sciences 2020 Feb 7;21(3)

The glucocorticoid receptor-FKBP51 complex contributes to fear conditioning and posttraumatic stress disorder.

Li H, Su P, Lai TK, Jiang A, Liu J, Zhai D, Campbell CT, Lee FH, Yong W, Pasricha S, Li S, Wong AH, Ressler KJ, Liu F
The Journal of clinical investigation 2020 Feb 3;130(2):877-889

Hypoxia regulates GR function through multiple mechanisms involving microRNAs 103 and 107.

Yang N, Berry A, Sauer C, Baxter M, Donaldson IJ, Forbes K, Donn R, Matthews L, Ray D
Molecular and cellular endocrinology 2020 Dec 1;518:111007

Corticosterone Excess-Mediated Mitochondrial Damage Induces Hippocampal Neuronal Autophagy in Mice Following Cold Exposure.

Xu B, Lang L, Li S, Yuan J, Wang J, Yang H, Lian S
Animals : an open access journal from MDPI 2019 Sep 14;9(9)

Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice.

Buck JM, O'Neill HC, Stitzel JA
Biochemical pharmacology 2019 Oct;168:438-451

Gestational stress induced differential expression of HDAC2 in male rat offspring hippocampus during development.

Liao L, Wang X, Yao X, Zhang B, Zhou L, Huang J
Neuroscience research 2019 Oct;147:9-16

Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet.

Quagliarini F, Mir AA, Balazs K, Wierer M, Dyar KA, Jouffe C, Makris K, Hawe J, Heinig M, Filipp FV, Barish GD, Uhlenhaut NH
Molecular cell 2019 Nov 21;76(4):531-545.e5

Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Wang D, Xu B, Wang J, Wang H, Guo J, Ji H, Li S, Wu R, Yang H, Lian S
Brain research 2019 Nov 1;1722:146354

An improved method for quantitative ChIP studies of nuclear receptor function.

Hunter AL, Narang N, Baxter M, Ray DW, Poolman TM
Journal of molecular endocrinology 2019 May 1;62(4):169-177

Cortisol Excess-Mediated Mitochondrial Damage Induced Hippocampal Neuronal Apoptosis in Mice Following Cold Exposure.

Xu B, Lang LM, Li SZ, Guo JR, Wang JF, Wang D, Zhang LP, Yang HM, Lian S
Cells 2019 Jun 18;8(6)

E47 modulates hepatic glucocorticoid action.

Hemmer MC, Wierer M, Schachtrup K, Downes M, Hübner N, Evans RM, Uhlenhaut NH
Nature communications 2019 Jan 18;10(1):306

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Lian S, Xu B, Wang D, Wang L, Li W, Yao R, Ji H, Wang J, Guo J, Li S, Yang H
Behavioural brain research 2019 Feb 1;359:304-311

Differences of physical vs. psychological stress: evidences from glucocorticoid receptor expression, hippocampal subfields injury, and behavioral abnormalities.

Li Y, Qin J, Yan J, Zhang N, Xu Y, Zhu Y, Sheng L, Zhu X, Ju S
Brain imaging and behavior 2019 Dec;13(6):1780-1788

REVERBa couples the circadian clock to hepatic glucocorticoid action.

Caratti G, Iqbal M, Hunter L, Kim D, Wang P, Vonslow RM, Begley N, Tetley AJ, Woodburn JL, Pariollaud M, Maidstone R, Donaldson IJ, Zhang Z, Ince LM, Kitchen G, Baxter M, Poolman TM, Daniels DA, Stirling DR, Brocker C, Gonzalez F, Loudon AS, Bechtold DA, Rattray M, Matthews LC, Ray DW
The Journal of clinical investigation 2018 Oct 1;128(10):4454-4471

l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway.

Liang M, Wang Z, Li H, Cai L, Pan J, He H, Wu Q, Tang Y, Ma J, Yang L
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 May;115:315-328

α-Viniferin activates autophagic apoptosis and cell death by reducing glucocorticoid receptor expression in castration-resistant prostate cancer cells.

Cheng K, Liu X, Chen L, Lv JM, Qu FJ, Pan XW, Li L, Cui XG, Gao Y, Xu DF
Medical oncology (Northwood, London, England) 2018 Jun 15;35(7):105

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Lian S, Wang D, Xu B, Guo W, Wang L, Li W, Ji H, Wang J, Kong F, Zhen L, Li S, Zhang L, Guo J, Yang H
Behavioural brain research 2018 Jul 2;346:1-10

Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats.

Zhao N, Yang S, Jia Y, Sun B, He B, Zhao R
The Journal of nutritional biochemistry 2018 Apr;54:105-112

A non-transcriptional role for the glucocorticoid receptor in mediating the cell stress response.

Ptushkina M, Poolman T, Iqbal M, Ashe M, Petersen J, Woodburn J, Rattray M, Whetton A, Ray D
Scientific reports 2017 Sep 21;7(1):12101

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

Zhong X, Tian S, Zhang X, Diao X, Dong F, Yang J, Li Z, Sun L, Wang L, He X, Wu G, Hu X, Wang L, Song L, Zhang H, Pan X, Li A, Gao P
EMBO reports 2017 May;18(5):809-825

Glucocorticoid therapy regulates podocyte motility by inhibition of Rac1.

McCaffrey JC, Webb NJ, Poolman TM, Fresquet M, Moxey C, Zeef LAH, Donaldson IJ, Ray DW, Lennon R
Scientific reports 2017 Jul 27;7(1):6725

Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.

Lian S, Guo J, Wang L, Li W, Wang J, Ji H, Kong F, Xu B, Li S, Yang H
Oncotarget 2017 Dec 29;8(70):115304-115314

Long non-coding RNA GAS5 controls human embryonic stem cell self-renewal by maintaining NODAL signalling.

Xu C, Zhang Y, Wang Q, Xu Z, Jiang J, Gao Y, Gao M, Kang J, Wu M, Xiong J, Ji K, Yuan W, Wang Y, Liu H
Nature communications 2016 Nov 4;7:13287

Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.

Li P, Zhang D, Shen L, Dong K, Wu M, Ou Z, Shi D
Scientific reports 2016 Mar 9;6:22831

HOXA13 exerts a beneficial effect in albumin-induced epithelial-mesenchymal transition via the glucocorticoid receptor signaling pathway in human renal tubular epithelial cells.

Peng L, He Q, Li X, Shuai L, Chen H, Li Y, Yi Z
Molecular medicine reports 2016 Jul;14(1):271-6

The secretion, synthesis, and metabolism of cortisol and its downstream genes in the H-P-I axis of rare minnows (Gobiocypris rarus) are disrupted by acute waterborne cadmium exposure.

Liu XH, Xie BW, Wang ZJ, Jin L, Zhang YG
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 2016 Jul-Aug;185-186:112-121

Glucocorticoid receptor regulates accurate chromosome segregation and is associated with malignancy.

Matthews LC, Berry AA, Morgan DJ, Poolman TM, Bauer K, Kramer F, Spiller DG, Richardson RV, Chapman KE, Farrow SN, Norman MR, Williamson AJ, Whetton AD, Taylor SS, Tuckermann JP, White MR, Ray DW
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 28;112(17):5479-84

Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet.

Hu YW, Ma X, Huang JL, Mao XR, Yang JY, Zhao JY, Li SF, Qiu YR, Yang J, Zheng L, Wang Q
PloS one 2013;8(6):e66876

Pin1 promotes GR transactivation by enhancing recruitment to target genes.

Poolman TM, Farrow SN, Matthews L, Loudon AS, Ray DW
Nucleic acids research 2013 Oct;41(18):8515-25

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 24050-1-AP(NR3C1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using NR3C1 antibody 24050-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line