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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- ELISA [1]
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- Product number
- 500-P120-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL6 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: VLTELRCTCL RVTLRVNPKT IGKLQVFPAG PQCSKVEVVA SLKNGKQVCL DPEAPFLKKV IQKILDSGNK KN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GCP-2 (CXCL6). Anti-Human GCP-2 (CXCL6) specific antibody was purified by affinity chromatography employing an immobilized Human GCP-2 matrix. Sandwich ELISA: To detect Human GCP-2 (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GCP-2 (CXCL6)(500-P120Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GCP-2 (CXCL6). Western Blot: To detect Human GCP-2 (CXCL6) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GCP-2 (CXCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Tumor angiogenesis induced by granulocyte chemotactic protein-2 as a countercurrent principle.
Van Coillie E, Van Aelst I, Wuyts A, Vercauteren R, Devos R, De Wolf-Peeters C, Van Damme J, Opdenakker G
The American journal of pathology 2001 Oct;159(4):1405-14
The American journal of pathology 2001 Oct;159(4):1405-14
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human GCP-2 (CXCL6) by Western Blot analysis CXCL6 Polyclonal Antibody (Product # 500-P120-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GCP-2 (CXCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human GCP-2 (CXCL6) by Western Blot analysis CXCL6 Polyclonal Antibody (Product # 500-P120-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GCP-2 (CXCL6) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human GCP-2 (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CXCL6 Polyclonal Antibody (Product # 500-P120-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CXCL6 Polyclonal Antibody, Biotin (Product # 500-P120BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GCP-2 (CXCL6).
