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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- AF254 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCL5/ENA-78 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CXCL5/ENA-78 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GCP-2 is observed and less than 5% cross-reactivity with recombinant mouse LIX, recombinant rat LIX and rhGRO gamma is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P42830- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment.
Neutrophil infiltration mediated by CXCL5 accumulation in the laryngeal squamous cell carcinoma microenvironment: A mechanism by which tumour cells escape immune surveillance.
Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-β.
Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer.
A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients.
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, Kuchimaru T, Sakamoto T, Watanabe H, Kondoh G, Kizaka-Kondoh S
Oncotarget 2018 Feb 16;9(13):11209-11226
Oncotarget 2018 Feb 16;9(13):11209-11226
Neutrophil infiltration mediated by CXCL5 accumulation in the laryngeal squamous cell carcinoma microenvironment: A mechanism by which tumour cells escape immune surveillance.
Zhang D, Zhou J, Tang D, Zhou L, Chou L, Chou KY, Tao L, Lu LM
Clinical immunology (Orlando, Fla.) 2017 Feb;175:34-40
Clinical immunology (Orlando, Fla.) 2017 Feb;175:34-40
Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
Stem cells (Dayton, Ohio) 2016 Apr;34(4):1112-22
Stem cells (Dayton, Ohio) 2016 Apr;34(4):1112-22
CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-β.
Jablonska J, Wu CF, Andzinski L, Leschner S, Weiss S
International journal of cancer 2014 Mar 15;134(6):1346-58
International journal of cancer 2014 Mar 15;134(6):1346-58
Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer.
Li A, King J, Moro A, Sugi MD, Dawson DW, Kaplan J, Li G, Lu X, Strieter RM, Burdick M, Go VL, Reber HA, Eibl G, Hines OJ
The American journal of pathology 2011 Mar;178(3):1340-9
The American journal of pathology 2011 Mar;178(3):1340-9
A CXCL5- and bFGF-dependent effect of PDGF-B-activated fibroblasts in promoting trafficking and differentiation of bone marrow-derived mesenchymal stem cells.
Nedeau AE, Bauer RJ, Gallagher K, Chen H, Liu ZJ, Velazquez OC
Experimental cell research 2008 Jul 1;314(11-12):2176-86
Experimental cell research 2008 Jul 1;314(11-12):2176-86
Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients.
Dimberg J, Dienus O, Löfgren S, Hugander A, Wågsäter D
International journal of oncology 2007 Jul;31(1):97-102
International journal of oncology 2007 Jul;31(1):97-102
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CXCL5/ENA-78 by Western Blot. Western blot shows conditioned media (CM) and lysates from A549 human lung carcinoma cell line untreated (-) or treated (+) with human IL-1 beta for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CXCL5/ENA-78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CXCL5/ENA-78 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL5/ENA-78 in Human Breast Cancer Tissue. CXCL5/ENA-78 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human CXCL5/ENA-78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL5/ENA-78 in Human Breast. CXCL5/ENA-78 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human CXCL5/ENA-78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL5/ENA-78 and Neutralization by Human CXCL5/ENA-78 Antibody. Recombinant Human CXCL5/ENA-78 (Catalog # 254-XB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL5/ENA-78 (0.03 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL5/ENA-78 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF254). The ND50 is typically 0.2-1.0 µg/mL.
