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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-64136 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LIMCH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LHEAYKNARS QEEAEGILQQ YIERFTISEA VLERLEMPKI LERSHSTEPN LSSFLNDPNP MKYLRQQSLP PPKFTATVET TIARASV
- Concentration
- 0.4 mg/mL
Submitted references Nup93 regulates breast tumor growth by modulating cell proliferation and actin cytoskeleton remodeling.
Bersini S, Lytle NK, Schulte R, Huang L, Wahl GM, Hetzer MW
Life science alliance 2020 Jan;3(1)
Life science alliance 2020 Jan;3(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LIMCH1 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG. Samples were probed using a LIMCH1 Polyclonal Antibody (Product # PA5-64136).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of LIMCH1 in human cell line HeLa using a LIMCH1 Polyclonal Antibody (Product # PA5-64136) shows localization to actin filaments.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of LIMCH1 in human kidney tissue shows moderate cytoplasmic and membranous positivity in cells in tubules and cells in glomeruli. Samples were probed using a LIMCH1 Polyclonal Antibody (Product # PA5-64136).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. Nup93 directly regulates genes associated with ECM/cytoskeleton remodeling, stress fiber formation, and cell migration. (A) Venn diagram correlating data from RNAseq and Cut&Run (protein-chromatin interactions). NUP93 KD up-regulates genes associated with stress fiber formation (e.g., LIMCH1 and EDN1 ). (B) Representative images of RNAseq and Cut&Run peaks showing direct regulation of specific genes (representative protein-chromatin interactions at different genomic regions). Tracks were visualized using the University of California Santa Cruz Genome Browser. Tracks of NUP93 KD and controls are normalized for direct comparison. (C, D, E, F) Simultaneous KD of NUP93 and LIMCH1 (C) partially restores cell migration compared with NUP93 KD cells (D) ( t test with P < 0.05, N >= 110 single cells tracked per condition in three biological replicates, data normalized to NUP93 KD cells) and partially impairs the formation of stress fibers (E, F). Representative images and intensity profiles. Scale bars: 10 mum.
