Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN950656 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Betacellulin (BTC) (N-Term), (AA 24-54) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human BTC
- Description
- Saturated Ammonium Sulfate (SAS) precipitation
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 24-54
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Sequential and gamma-secretase-dependent processing of the betacellulin precursor generates a palmitoylated intracellular-domain fragment that inhibits cell growth.
Betacellulin inhibits osteogenic differentiation and stimulates proliferation through HIF-1alpha.
A betacellulin mutant promotes differentiation of pancreatic acinar AR42J cells into insulin-producing cells with low affinity of binding to ErbB1.
ErbB/HER ligands in human breast cancer, and relationships with their receptors, the bio-pathological features and prognosis.
The ADAM10 prodomain is a specific inhibitor of ADAM10 proteolytic activity and inhibits cellular shedding events.
Stoeck A, Shang L, Dempsey PJ
Journal of cell science 2010 Jul 1;123(Pt 13):2319-31
Journal of cell science 2010 Jul 1;123(Pt 13):2319-31
Betacellulin inhibits osteogenic differentiation and stimulates proliferation through HIF-1alpha.
Genetos DC, Rao RR, Vidal MA
Cell and tissue research 2010 Apr;340(1):81-9
Cell and tissue research 2010 Apr;340(1):81-9
A betacellulin mutant promotes differentiation of pancreatic acinar AR42J cells into insulin-producing cells with low affinity of binding to ErbB1.
Nagaoka T, Fukuda T, Hashizume T, Nishiyama T, Tada H, Yamada H, Salomon DS, Yamada S, Kojima I, Seno M
Journal of molecular biology 2008 Jun 27;380(1):83-94
Journal of molecular biology 2008 Jun 27;380(1):83-94
ErbB/HER ligands in human breast cancer, and relationships with their receptors, the bio-pathological features and prognosis.
RĂ©villion F, Lhotellier V, Hornez L, Bonneterre J, Peyrat JP
Annals of oncology : official journal of the European Society for Medical Oncology / ESMO 2008 Jan;19(1):73-80
Annals of oncology : official journal of the European Society for Medical Oncology / ESMO 2008 Jan;19(1):73-80
The ADAM10 prodomain is a specific inhibitor of ADAM10 proteolytic activity and inhibits cellular shedding events.
Moss ML, Bomar M, Liu Q, Sage H, Dempsey P, Lenhart PM, Gillispie PA, Stoeck A, Wildeboer D, Bartsch JW, Palmisano R, Zhou P
The Journal of biological chemistry 2007 Dec 7;282(49):35712-21
The Journal of biological chemistry 2007 Dec 7;282(49):35712-21
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- BTC Antibody (N-term) (Cat. #AP50402PU-N) western blot analysis in MDA-MB231 cell line lysates (35μg/lane).This demonstrates the BTC antibody detected the BTC protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of BTC Antibody (N-term)(Cat#AP50402PU-N) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- BTC Antibody (N-term) (Cat. #AP50402PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- BTC Antibody (N-term) (Cat. #AP50402PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.