ABIN950656

antibody from antibodies-online

Targeting: BTC

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN950656

Provider

antibodies-online

Product name

anti-Betacellulin (BTC) (N-Term), (AA 24-54) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human BTC

Description

Saturated Ammonium Sulfate (SAS) precipitation

Reactivity

Human

Host

Rabbit

Epitope

N-Term,AA 24-54

Vial size

0.4 mL

Concentration

0.25 mg/mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Sequential and gamma-secretase-dependent processing of the betacellulin precursor generates a palmitoylated intracellular-domain fragment that inhibits cell growth.

Stoeck A, Shang L, Dempsey PJ
Journal of cell science 2010 Jul 1;123(Pt 13):2319-31

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Betacellulin inhibits osteogenic differentiation and stimulates proliferation through HIF-1alpha.

Genetos DC, Rao RR, Vidal MA
Cell and tissue research 2010 Apr;340(1):81-9

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A betacellulin mutant promotes differentiation of pancreatic acinar AR42J cells into insulin-producing cells with low affinity of binding to ErbB1.

Nagaoka T, Fukuda T, Hashizume T, Nishiyama T, Tada H, Yamada H, Salomon DS, Yamada S, Kojima I, Seno M
Journal of molecular biology 2008 Jun 27;380(1):83-94

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ErbB/HER ligands in human breast cancer, and relationships with their receptors, the bio-pathological features and prognosis.

Révillion F, Lhotellier V, Hornez L, Bonneterre J, Peyrat JP
Annals of oncology : official journal of the European Society for Medical Oncology / ESMO 2008 Jan;19(1):73-80

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The ADAM10 prodomain is a specific inhibitor of ADAM10 proteolytic activity and inhibits cellular shedding events.

Moss ML, Bomar M, Liu Q, Sage H, Dempsey P, Lenhart PM, Gillispie PA, Stoeck A, Wildeboer D, Bartsch JW, Palmisano R, Zhou P
The Journal of biological chemistry 2007 Dec 7;282(49):35712-21

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

BTC Antibody (N-term) (Cat. #AP50402PU-N) western blot analysis in MDA-MB231 cell line lysates (35μg/lane).This demonstrates the BTC antibody detected the BTC protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of BTC Antibody (N-term)(Cat#AP50402PU-N) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

BTC Antibody (N-term) (Cat. #AP50402PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

BTC Antibody (N-term) (Cat. #AP50402PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.