Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 16276-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- G3BP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LLSNSGQPE RKFMQTFVLA PEGSVPNKFY VHNDMFRYED EVFGDSEPEL DEESEDEVEE EQEERQPSPE PVQENANSGY YEAHPVTNGI EEPLEESSHE PEPEPESETK TEELKPQVEE KNLEELEEKS TTPPPAEPVS LPQEPPKPRV EAKPEVQSQP PRVREQRPRE RPGFPPRGPR PGRGDMEQND SDNRRIIRYP DSHQLFVGNL PHDIDENELK EFFMSFGNVV ELRINTKGVG GKLPNFGFVV FDDSEPVQRI LIAKPIMFRG EVRLNVEEKK TRAARERETR GGGDDRRDIR RNDRGPGGPR GIVGGGMMRD RDGRGPPPRG GMAQKLGSGR GTGQMEGRFT GQRR (97-449 aa encoded by BC011731)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of G3BP2 antibody (16276-1-AP, 1:500) with T47D breast cancer cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP ( G3BP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 16276-1-AP (G3BP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (Product # 16276-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 16276-1-AP (G3BP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 16276-1-AP ( G3BP2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 16276-1-AP ( G3BP2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-G3BP2 (IP:16276-1-AP, 4ug; Detection:16276-1-AP 1:1000) with HeLa cells lysate 2200 ug.