NB400-128

antibody from Novus Biologicals

Targeting: NPC1L1 SLC65A2

Provider product page for NB400-128

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [22]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: NB400-128 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB400-128, RRID:AB_10000815
Product name: Rabbit Polyclonal Niemann-Pick type C1 Like-1 Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

NPC1L1 protein structure - NB400-128 shown in red.

Submitted references Hepatic Expression of Niemann-Pick C1-Like 1, a Cholesterol Reabsorber from Bile, Exacerbates Western Diet-Induced Atherosclerosis in LDL Receptor Mutant Mice.

Yamamoto H, Yamanashi Y, Takada T, Mu S, Tanaka Y, Komine T, Suzuki H
Molecular pharmacology 2019 Jul;96(1):47-55

Duodenal Niemann-Pick C1-like 1 expression was negatively correlated with liver X receptor expression in nonalcoholic fatty liver disease.

Ahn SB, Jun DW, Jang K, Lee BH, Shin KJ
The Korean journal of internal medicine 2019 Jul;34(4):777-784

Lipid-lowering effects of Coffea arabica pulp aqueous extract in Caco-2 cells and hypercholesterolemic rats.

Ontawong A, Duangjai A, Muanprasat C, Pasachan T, Pongchaidecha A, Amornlerdpison D, Srimaroeng C
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 Jan;52:187-197

The atypical antipsychotic quetiapine induces hyperlipidemia by activating intestinal PXR signaling.

Meng Z, Gwag T, Sui Y, Park SH, Zhou X, Zhou C
JCI insight 2019 Feb 7;4(3)

Pathophysiological importance of bile cholesterol reabsorption: hepatic NPC1L1-exacerbated steatosis and decreasing VLDL-TG secretion in mice fed a high-fat diet.

Toyoda Y, Takada T, Yamanashi Y, Suzuki H
Lipids in health and disease 2019 Dec 28;18(1):234

Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane.

Soayfane Z, Tercé F, Cantiello M, Robenek H, Nauze M, Bézirard V, Allart S, Payré B, Capilla F, Cartier C, Peres C, Al Saati T, Théodorou V, Nelson DW, Yen CE, Collet X, Coméra C
Nutrition & metabolism 2016;13:48

Pravastatin Modulate Niemann-Pick C1-Like 1 and ATP-Binding Cassette G5 and G8 to Influence Intestinal Cholesterol Absorption.

Kawase A, Hata S, Takagi M, Iwaki M
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques 2015;18(5):765-72

Pravastatin Modulate Niemann-Pick C1-Like 1 and ATP-Binding Cassette G5 and G8 to Influence Intestinal Cholesterol Absorption.

New Insights In Intestinal Sar1B GTPase Regulation and Role in Cholesterol Homeostasis.

Sané A, Seidman E, Spahis S, Lamantia V, Garofalo C, Montoudis A, Marcil V, Levy E
Journal of cellular biochemistry 2015 Oct;116(10):2270-82

Intestinal pregnane X receptor links xenobiotic exposure and hypercholesterolemia.

Sui Y, Helsley RN, Park SH, Song X, Liu Z, Zhou C
Molecular endocrinology (Baltimore, Md.) 2015 May;29(5):765-76

NPC1L1 is a key regulator of intestinal vitamin K absorption and a modulator of warfarin therapy.

Takada T, Yamanashi Y, Konishi K, Yamamoto T, Toyoda Y, Masuo Y, Yamamoto H, Suzuki H
Science translational medicine 2015 Feb 18;7(275):275ra23

Expression of liver X receptor correlates with intrahepatic inflammation and fibrosis in patients with nonalcoholic fatty liver disease.

Ahn SB, Jang K, Jun DW, Lee BH, Shin KJ
Digestive diseases and sciences 2014 Dec;59(12):2975-82

D-Glucose modulates intestinal Niemann-Pick C1-like 1 (NPC1L1) gene expression via transcriptional regulation.

Malhotra P, Boddy CS, Soni V, Saksena S, Dudeja PK, Gill RK, Alrefai WA
American journal of physiology. Gastrointestinal and liver physiology 2013 Jan 15;304(2):G203-10

Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 from fermented foods.

Yoon HS, Ju JH, Kim HN, Park HJ, Ji Y, Lee JE, Shin HK, Do MS, Holzapfel W
International journal of food sciences and nutrition 2013 Feb;64(1):44-52

EP 80317, a CD36 selective ligand, promotes reverse cholesterol transport in apolipoprotein E-deficient mice.

Bujold K, Mellal K, Zoccal KF, Rhainds D, Brissette L, Febbraio M, Marleau S, Ong H
Atherosclerosis 2013 Aug;229(2):408-14

Black pepper and piperine reduce cholesterol uptake and enhance translocation of cholesterol transporter proteins.

Duangjai A, Ingkaninan K, Praputbut S, Limpeanchob N
Journal of natural medicines 2013 Apr;67(2):303-10

PCSK9 plays a significant role in cholesterol homeostasis and lipid transport in intestinal epithelial cells.

Levy E, Ben Djoudi Ouadda A, Spahis S, Sane AT, Garofalo C, Grenier É, Emonnot L, Yara S, Couture P, Beaulieu JF, Ménard D, Seidah NG, Elchebly M
Atherosclerosis 2013 Apr;227(2):297-306

Lecithin:cholesterol acyltransferase deficiency protects against cholesterol-induced hepatic endoplasmic reticulum stress in mice.

Hager L, Li L, Pun H, Liu L, Hossain MA, Maguire GF, Naples M, Baker C, Magomedova L, Tam J, Adeli K, Cummins CL, Connelly PW, Ng DS
The Journal of biological chemistry 2012 Jun 8;287(24):20755-68

Mutation in EGFP domain of LDL receptor-related protein 6 impairs cellular LDL clearance.

Liu W, Mani S, Davis NR, Sarrafzadegan N, Kavathas PB, Mani A
Circulation research 2008 Nov 21;103(11):1280-8

Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake.

Montoudis A, Seidman E, Boudreau F, Beaulieu JF, Menard D, Elchebly M, Mailhot G, Sane AT, Lambert M, Delvin E, Levy E
Journal of lipid research 2008 May;49(5):961-72

Purified NPC1 protein. I. Binding of cholesterol and oxysterols to a 1278-amino acid membrane protein.

Infante RE, Abi-Mosleh L, Radhakrishnan A, Dale JD, Brown MS, Goldstein JL
The Journal of biological chemistry 2008 Jan 11;283(2):1052-63

CD36 is important for fatty acid and cholesterol uptake by the proximal but not distal intestine.

Nassir F, Wilson B, Han X, Gross RW, Abumrad NA
The Journal of biological chemistry 2007 Jul 6;282(27):19493-501

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Detection of NPC3 in rat small intestine membrane preparations (20 ug). (1) 2 ug/ml and (2) 4 ug/ml. ECL: 30 minute exposure.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NB400-128] - Staining of human small intestine, enterocytes.