Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 10899-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EIF3E Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SGAAEYLYF FRVLVPATDR NALSSLWGKL ASEILMQNWD AAMEDLTRLK ETIDNNSVSS PLQSLQQRTW LIHWSLFVFF NHPKGRDNII DLFLYQPQYL NAIQTMCPHI LRYLTTAVIT NKDVRKRRQV LKDLVKVIQQ ESYTYKDPIT EFVECLYVNF DFDGAQKKLR ECESVLVNDF FLVACLEDFI ENARLFIFET FCRIHQCISI NMLADKLNMT PEEAERWIVN LIRNARLDAK IDSKLGHVVM GNNAVSPYQQ VIEKTKSLSF RSQMLAMNIE KKLNQNSRSE APNWATQDSG FY (145-445 aa encoded by BC008419)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat lymph tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SH-SY5Y cells, using EIF3E antibody 10899-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 10899-1-AP (EIF3E antibody) at dilution of 1:100 (under 10x lens).
