GTX48712

antibody from GeneTex

Targeting: EIF3E eIF3-p48, EIF3S6, INT6

ELISA

Antibody data

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Validation data

EIF3E protein structure - GTX48712 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot using GeneTex's affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral shRNA knockdown vectors
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Provider: Customized siRNA
KD Reagent Prod page: http://www.genetex.com
KD Reagent Prod no: GTX48712
Antibody Lot Number: 39224

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot using GeneTex's affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blot using GeneTex's affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000.