MAB554-100

antibody from R&D Systems

Targeting: CD4

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

MAB554-100

Provider

R&D Systems

Product name

Mouse CD4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

GK1.5

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Interleukin-12p35 Deficiency Reverses the Th1/Th2 Imbalance, Aggravates the Th17/Treg Imbalance, and Ameliorates Atherosclerosis in ApoE-/- Mice.

Huang Y, Hu H, Liu L, Ye J, Wang Z, Que B, Liu W, Shi Y, Zeng T, Shi L, Ji Q, Chang C, Lin Y
Mediators of inflammation 2019;2019:3152040

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Hypoxic pre-conditioning suppresses experimental autoimmune encephalomyelitis by modifying multiple properties of blood vessels.

Halder SK, Kant R, Milner R
Acta neuropathologica communications 2018 Sep 3;6(1):86

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The efficacy of combination therapy with oncolytic herpes simplex virus HF10 and dacarbazine in a mouse melanoma model.

Tanaka R, Goshima F, Esaki S, Sato Y, Murata T, Nishiyama Y, Watanabe D, Kimura H
American journal of cancer research 2017;7(8):1693-1703

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Combination of CD40 Agonism and CSF-1R Blockade Reconditions Tumor-Associated Macrophages and Drives Potent Antitumor Immunity.

Wiehagen KR, Girgis NM, Yamada DH, Smith AA, Chan SR, Grewal IS, Quigley M, Verona RI
Cancer immunology research 2017 Dec;5(12):1109-1121

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PAX7 expression defines germline stem cells in the adult testis.

Aloisio GM, Nakada Y, Saatcioglu HD, Peña CG, Baker MD, Tarnawa ED, Mukherjee J, Manjunath H, Bugde A, Sengupta AL, Amatruda JF, Cuevas I, Hamra FK, Castrillon DH
The Journal of clinical investigation 2014 Sep;124(9):3929-44

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Oncolytic viral therapy with a combination of HF10, a herpes simplex virus type 1 variant and granulocyte-macrophage colony-stimulating factor for murine ovarian cancer.

Goshima F, Esaki S, Luo C, Kamakura M, Kimura H, Nishiyama Y
International journal of cancer 2014 Jun 15;134(12):2865-77

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Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.

Mills JH, Alabanza LM, Mahamed DA, Bynoe MS
Journal of neuroinflammation 2012 Aug 10;9:193

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Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide.

Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO
The Journal of investigative dermatology 2006 Aug;126(8):1792-803

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CD4 in Mouse Intestine. CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Catalog # MAB554) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.