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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3535 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3535, RRID:AB_1572332
- Product name
- CD4 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 408-436 at C-terminus of human CD4.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400 µl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Structural basis for the interaction between focal adhesion kinase and CD4.
Sequence variation occurs in CD4 epitopes during early HIV infection.
Garron ML, Arthos J, Guichou JF, McNally J, Cicala C, Arold ST
Journal of molecular biology 2008 Feb 1;375(5):1320-8
Journal of molecular biology 2008 Feb 1;375(5):1320-8
Sequence variation occurs in CD4 epitopes during early HIV infection.
Rychert J, Saindon S, Placek S, Daskalakis D, Rosenberg E
Journal of acquired immune deficiency syndromes (1999) 2007 Nov 1;46(3):261-7
Journal of acquired immune deficiency syndromes (1999) 2007 Nov 1;46(3):261-7
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CD4 polyclonal antibody (Cat # PAB3535) in mouse heart tissue lysates (35 ug/lane).CD4 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Validation comment
- Western Blot (Tissue lysate)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human tonsil reacted with CD4 polyclonal antibody (Cat # PAB3535), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CEM cells using CD4 polyclonal antibody (Cat # PAB3535)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
