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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [24]
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- Validations
- Western blot [4]
- Immunohistochemistry [5]
- Flow cytometry [2]
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- Product number
- NBP1-19371 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-19371, RRID:AB_1641682
- Product name
- Rabbit Polyclonal CD4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Canine, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references High-Frequency Irreversible Electroporation for Treatment of Primary Liver Cancer: A Proof-of-Principle Study in Canine Hepatocellular Carcinoma.
Mesenchymal Stem Cells Decrease Oxidative Stress in the Bowels of Interleukin-10 Knockout Mice.
New insights on repeated acoustic injury: Augmentation of cochlear susceptibility and inflammatory reaction resultant of prior acoustic injury.
Evaluation of Anti-Tumor Effects of Whole-Body Low-Dose Irradiation in Metastatic Mouse Models.
Trafficking of immune cells across the blood-brain barrier is modulated by neurofibrillary pathology in tauopathies.
Cardiac troponin I autoantibody induces myocardial dysfunction by PTEN signaling activation.
Characterization of inflammatory infiltrate of ulcerative dermatitis in C57BL/6NCrl-Tg(HMGA1P6)1Pg mice.
High fat diet downregulates regulatory T cells in the myocardium of spontaneous hypertensive rats.
Age-Related Dopaminergic Innervation Augments T Helper 2-Type Allergic Inflammation in the Postnatal Lung.
Immunological and inflammatory mapping of vascularized composite allograft rejection processes in a rat model.
Quercetin protects against atherosclerosis by inhibiting dendritic cell activation.
The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis.
Prime-boost immunization by both DNA vaccine and oncolytic adenovirus expressing GM-CSF and shRNA of TGF-β2 induces anti-tumor immune activation.
5-aza-2'-deoxycytidine impairs mouse spermatogenesis at multiple stages through different usage of DNA methyltransferases.
Absence of the intestinal microbiota exacerbates hepatobiliary disease in a murine model of primary sclerosing cholangitis.
Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjögren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model.
Activation of the antigen presentation function of mononuclear phagocyte populations associated with the basilar membrane of the cochlea after acoustic overstimulation.
Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice.
Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma.
CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited.
Notch γ-secretase inhibitor dibenzazepine attenuates angiotensin II-induced abdominal aortic aneurysm in ApoE knockout mice by multiple mechanisms.
Simultaneous blockade of programmed death 1 and vascular endothelial growth factor receptor 2 (VEGFR2) induces synergistic anti-tumour effect in vivo.
The effect of programmed cryopreservation on immunogenicity of bladder mucosa in New Zealand rabbits.
An acidic polysaccharide of Panax ginseng ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.
Partridge BR, O'Brien TJ, Lorenzo MF, Coutermarsh-Ott SL, Barry SL, Stadler K, Muro N, Meyerhoeffer M, Allen IC, Davalos RV, Dervisis NG
Journal of vascular and interventional radiology : JVIR 2020 Mar;31(3):482-491.e4
Journal of vascular and interventional radiology : JVIR 2020 Mar;31(3):482-491.e4
Mesenchymal Stem Cells Decrease Oxidative Stress in the Bowels of Interleukin-10 Knockout Mice.
Jung KJ, Lee GW, Park CH, Lee TJ, Kim JY, Sung EG, Kim SY, Jang BI, Song IH
Gut and liver 2020 Jan 15;14(1):100-107
Gut and liver 2020 Jan 15;14(1):100-107
New insights on repeated acoustic injury: Augmentation of cochlear susceptibility and inflammatory reaction resultant of prior acoustic injury.
Zhang C, Frye MD, Sun W, Sharma A, Manohar S, Salvi R, Hu BH
Hearing research 2020 Aug;393:107996
Hearing research 2020 Aug;393:107996
Evaluation of Anti-Tumor Effects of Whole-Body Low-Dose Irradiation in Metastatic Mouse Models.
Song KH, Jung SY, Park JI, Ahn J, Park JK, Hwang SG, Kim EH, Nam SY, Park S, Ha H, Song JY
Cancers 2020 Apr 30;12(5)
Cancers 2020 Apr 30;12(5)
Trafficking of immune cells across the blood-brain barrier is modulated by neurofibrillary pathology in tauopathies.
Majerova P, Michalicova A, Cente M, Hanes J, Vegh J, Kittel A, Kosikova N, Cigankova V, Mihaljevic S, Jadhav S, Kovac A
PloS one 2019;14(5):e0217216
PloS one 2019;14(5):e0217216
Cardiac troponin I autoantibody induces myocardial dysfunction by PTEN signaling activation.
Wu Y, Qin YH, Liu Y, Zhu L, Zhao XX, Liu YY, Luo SW, Tang GS, Shen Q
EBioMedicine 2019 Sep;47:329-340
EBioMedicine 2019 Sep;47:329-340
Characterization of inflammatory infiltrate of ulcerative dermatitis in C57BL/6NCrl-Tg(HMGA1P6)1Pg mice.
De Biase D, Esposito F, De Martino M, Pirozzi C, Luciano A, Palma G, Raso GM, Iovane V, Marzocco S, Fusco A, Paciello O
Laboratory animals 2019 Oct;53(5):447-458
Laboratory animals 2019 Oct;53(5):447-458
High fat diet downregulates regulatory T cells in the myocardium of spontaneous hypertensive rats.
Hong SK, Choo EH, Ihm SH, Chang KY
Nutrition, metabolism, and cardiovascular diseases : NMCD 2019 Nov;29(11):1254-1260
Nutrition, metabolism, and cardiovascular diseases : NMCD 2019 Nov;29(11):1254-1260
Age-Related Dopaminergic Innervation Augments T Helper 2-Type Allergic Inflammation in the Postnatal Lung.
Wang W, Cohen JA, Wallrapp A, Trieu KG, Barrios J, Shao F, Krishnamoorthy N, Kuchroo VK, Jones MR, Fine A, Bai Y, Ai X
Immunity 2019 Dec 17;51(6):1102-1118.e7
Immunity 2019 Dec 17;51(6):1102-1118.e7
Immunological and inflammatory mapping of vascularized composite allograft rejection processes in a rat model.
Friedman O, Carmel N, Sela M, Abu Jabal A, Inbal A, Ben Hamou M, Krelin Y, Gur E, Shani N
PloS one 2017;12(7):e0181507
PloS one 2017;12(7):e0181507
Quercetin protects against atherosclerosis by inhibiting dendritic cell activation.
Lin W, Wang W, Wang D, Ling W
Molecular nutrition & food research 2017 Sep;61(9)
Molecular nutrition & food research 2017 Sep;61(9)
The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis.
Liu S, Pei F, Wang X, Li D, Zhao L, Song Y, Chen Z, Liu B
Oncotarget 2017 Sep 12;8(39):66528-66539
Oncotarget 2017 Sep 12;8(39):66528-66539
Prime-boost immunization by both DNA vaccine and oncolytic adenovirus expressing GM-CSF and shRNA of TGF-β2 induces anti-tumor immune activation.
Kim SY, Kang D, Choi HJ, Joo Y, Kim JH, Song JJ
Oncotarget 2017 Feb 28;8(9):15858-15877
Oncotarget 2017 Feb 28;8(9):15858-15877
5-aza-2'-deoxycytidine impairs mouse spermatogenesis at multiple stages through different usage of DNA methyltransferases.
Song N, Endo D, Song B, Shibata Y, Koji T
Toxicology 2016 Jun 15;361-362:62-72
Toxicology 2016 Jun 15;361-362:62-72
Absence of the intestinal microbiota exacerbates hepatobiliary disease in a murine model of primary sclerosing cholangitis.
Tabibian JH, O'Hara SP, Trussoni CE, Tietz PS, Splinter PL, Mounajjed T, Hagey LR, LaRusso NF
Hepatology (Baltimore, Md.) 2016 Jan;63(1):185-96
Hepatology (Baltimore, Md.) 2016 Jan;63(1):185-96
Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjögren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model.
Gervais EM, Desantis KA, Pagendarm N, Nelson DA, Enger T, Skarstein K, Liaaen Jensen J, Larsen M
Anatomical record (Hoboken, N.J. : 2007) 2015 Sep;298(9):1622-34
Anatomical record (Hoboken, N.J. : 2007) 2015 Sep;298(9):1622-34
Activation of the antigen presentation function of mononuclear phagocyte populations associated with the basilar membrane of the cochlea after acoustic overstimulation.
Yang W, Vethanayagam RR, Dong Y, Cai Q, Hu BH
Neuroscience 2015 Sep 10;303:1-15
Neuroscience 2015 Sep 10;303:1-15
Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice.
Ren H, Li F, Tian C, Nie H, Wang L, Li HH, Zheng Y
Scientific reports 2015 Oct 28;5:15730
Scientific reports 2015 Oct 28;5:15730
Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma.
Migita K, Sho M, Shimada K, Yasuda S, Yamato I, Takayama T, Matsumoto S, Wakatsuki K, Hotta K, Tanaka T, Ito M, Konishi N, Nakajima Y
Cancer 2014 Mar 15;120(6):808-17
Cancer 2014 Mar 15;120(6):808-17
CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited.
Helsby MA, Leader PM, Fenn JR, Gulsen T, Bryant C, Doughton G, Sharpe B, Whitley P, Caunt CJ, James K, Pope AD, Kelly DH, Chalmers AD
BMC cell biology 2014 Feb 14;15:6
BMC cell biology 2014 Feb 14;15:6
Notch γ-secretase inhibitor dibenzazepine attenuates angiotensin II-induced abdominal aortic aneurysm in ApoE knockout mice by multiple mechanisms.
Zheng YH, Li FD, Tian C, Ren HL, Du J, Li HH
PloS one 2013;8(12):e83310
PloS one 2013;8(12):e83310
Simultaneous blockade of programmed death 1 and vascular endothelial growth factor receptor 2 (VEGFR2) induces synergistic anti-tumour effect in vivo.
Yasuda S, Sho M, Yamato I, Yoshiji H, Wakatsuki K, Nishiwada S, Yagita H, Nakajima Y
Clinical and experimental immunology 2013 Jun;172(3):500-6
Clinical and experimental immunology 2013 Jun;172(3):500-6
The effect of programmed cryopreservation on immunogenicity of bladder mucosa in New Zealand rabbits.
Lu Y, Li B, Wang X, Zheng J, Pu J, Ding Q, Li L, Xu K, Gao P, Chen L
Cryobiology 2012 Feb;64(1):27-32
Cryobiology 2012 Feb;64(1):27-32
An acidic polysaccharide of Panax ginseng ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.
Hwang I, Ahn G, Park E, Ha D, Song JY, Jee Y
Immunology letters 2011 Aug 30;138(2):169-78
Immunology letters 2011 Aug 30;138(2):169-78
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD4 Antibody [NBP1-19371] - Mouse spleen, 25ug total protein. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CD4 Antibody [NBP1-19371] - Lane view shows a specific band for CD4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD4 Antibody [NBP1-19371] - Dog spleen homogenate. Probed with anti-CD4 and anti-CD8. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Staining of paraffin-embedded mouse brain tissue. Photo courtesy of product review by verified customer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong signal in the membranes of a subset of cells in the spleen section and the staining showed punctate appearance in some cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE tissue section of mouse brain using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody primarily generated a strong signal in the membranes of a subset of cells in the tested section.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
