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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [20]
- Other assay [1]
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- Product number
- UM800049 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800049, RRID:AB_2629160
- Product name
- DDX56 mouse monoclonal antibody,clone UMAB152
- Antibody type
- Monoclonal
- Description
- DDX56 mouse monoclonal antibody,clone UMAB152
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- DDX56
- Isotype
- IgG
- Antibody clone number
- UMAB152
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of? HCT116 cell lysate (35ug) by using anti-DDX56 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of mouse tissue lysates (20ug) from Spleen. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot? Ultra (1:1000).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-DDX56 mouse monoclonal antibody (UM800049, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20?m.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-DDX56 mouse monoclonal antibody. (UM800049; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-DDX56 mouse monoclonal antibody (UM800049). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
