Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-22341 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP F Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (98%), Rat (98%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.45 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP F using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a hnRNP F polyclonal antibody (Product # PA5-22341) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using hnRNP F Polyclonal Antibody (Product # PA5-22341). HepG2 whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP F Polyclonal Antibody (Product # PA5-22341) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of hnRNP F in paraformaldehyde-fixed A431 cells using a hnRNP F polyclonal antibody (Product # PA5-22341) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- hnRNP F Polyclonal Antibody detects hnRNP F protein at nucleus on mouse uterus by immunohistochemical analysis. Sample: Paraffin-embedded mouse uterus. HnRNP F Polyclonal Antibody (Product # PA5-22341) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 Xenograft , using hnRNP F (Product # PA5-22341) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of hnRNP F was performed in 293T whole cell extracts using 5 µg of hnRNP F Polyclonal Antibody (Product # PA5-22341). Samples were transferred to a membrane and probed with hnRNP F Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.