12255-1-AP

antibody from Invitrogen Antibodies

Targeting: EBAG9 EB9, RCAS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12255-1-AP

Provider

Invitrogen Antibodies

Product name

RCAS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAITQFRLF KFCTCLATVF SFLKRLICRS GRGRKLSGDQ ITLPTTVDYS SVPKQTDVEE WTSWDEDAPT SVKIEGGNGN VATQQNSLEQ LEPDYFKDMT PTIRKTQKIV IKKREPLNFG IPDGSTGFSS RLAATQDLPF IHQSSELGDL DTWQENTNAW EEEEDAAWQA EEVLRQQKLA DREKRAAEQQ RKKMEKEAQR LMKKEQNKIG VKLS (1-213 aa encoded by BC017729)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 12255-1-AP ( RCAS1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12255-1-AP (RCAS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of RCAS1 antibody (12255-1-AP, 1:10000) with si-control and si-RCAS1 transfected HEK293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 12255-1-AP (RCAS1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using RCAS1 antibody (Product # 12255-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 12255-1-AP (RCAS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12255-1-AP ( RCAS1 antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12255-1-AP ( RCAS1 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RCAS1 (IP:12255-1-AP, 3ug; Detection:12255-1-AP 1:800) with mouse heart tissue lysate 1000ug.