66170-1-IG

antibody from Invitrogen Antibodies

Targeting: EBAG9 EB9, RCAS1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

66170-1-IG

Provider

Invitrogen Antibodies

Product name

RCAS1 Monoclonal Antibody (4H8A12)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MAITQFRLF KFCTCLATVF SFLKRLICRS GRGRKLSGDQ ITLPTTVDYS SVPKQTDVEE WTSWDEDAPT SVKIEGGNGN VATQQNSLEQ LEPDYFKDMT PTIRKTQKIV IKKREPLNFG IPDGSTGFSS RLAATQDLPF IHQSSELGDL DTWQENTNAW EEEEDAAWQA EEVLRQQKLA DREKRAAEQQ RKKMEKEAQR LMKKEQNKIG VKLS (1-213 aa encoded by BC017729)

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

4H8A12

Vial size

150 µL

Concentration

0.67 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66170-1-IG ( RCAS1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH 6.0.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66170-1-Ig (RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH 6.0.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66170-1-IG ( RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66170-1-IG ( RCAS1 antibody at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Jurkat cells were intracellularly stained with 0.2 µg Anti-Human RCAS1 (Product # 66170-1-IG, Clone:4H8A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Mouse IgG1 Isotype Control (66360-1-IG, Clone: T1F8D3F10) (black). Cells were fixed with 90% MeOH.