Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX104698 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104698, RRID:AB_1240674
- Product name
- Cyclophilin A antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Hamster
- Host
- Rabbit
Submitted references Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells.
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Lin CY, Chang TW, Hsieh WH, Hung MC, Lin IH, Lai SC, Tzeng YJ
Cell death discovery 2016;2:16065
Cell death discovery 2016;2:16065
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
May EW, Lin ST, Lin CC, Chang JF, Hung E, Lo YW, Lin LH, Hu RY, Feng CL, Lin DY, Wu SB, Lee WC, Lyu KW, Chou HC, Chan HL
Pharmacological research 2014 Dec;90:1-17
Pharmacological research 2014 Dec;90:1-17
Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Huang HJ, Lin CC, Chou HC, Chen YW, Lin ST, Lin YC, Lin DY, Lyu KW, Chan HL
Molecular bioSystems 2014 Dec;10(12):3086-100
Molecular bioSystems 2014 Dec;10(12):3086-100
Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Fan CY, Chou HC, Lo YW, Wen YF, Tsai YC, Huang H, Chan HL
Electrophoresis 2013 Dec;34(24):3305-14
Electrophoresis 2013 Dec;34(24):3305-14
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:H1299 B:HeLa S3(GTX14654)12% SDS PAGEGTX104698 diluted at 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cyclophilin A antibody detects Cyclophilin A protein by western blot analysis. Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody (GTX104698) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE GTX104698 diluted at 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cyclophilin A antibody detects Cyclophilin A protein by Western blot analysis. Mouse tissue extracts (50 £gg) was separated by 15 % SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody (GTX104698) at a dilution of 1:20000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Cyclophilin A(GTX104698) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Cyclophilin A(GTX104698) antibody at 1:500 dilution.