GTX100443

antibody from GeneTex

Targeting: CDH1 CD324, UVO, uvomorulin

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX100443

Provider

GeneTex

Proper citation

GeneTex Cat#GTX100443, RRID:AB_10729586

Product name

E-Cadherin antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat, Zebrafish

Host

Rabbit

References

Submitted references

Sympathetic innervation contributes to perineural invasion of salivary adenoid cystic carcinoma via the β2-adrenergic receptor.

Ma C, Gao T, Ju J, Zhang Y, Ni Q, Li Y, Zhao Z, Chai J, Yang X, Sun M
OncoTargets and therapy 2019;12:1475-1495

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ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.

DaSilva-Arnold SC, Kuo CY, Davra V, Remache Y, Kim PCW, Fisher JP, Zamudio S, Al-Khan A, Birge RB, Illsley NP
Molecular human reproduction 2019 Feb 1;25(2):61-75

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Prickle1 is required for EMT and migration of zebrafish cranial neural crest.

Ahsan K, Singh N, Rocha M, Huang C, Prince VE
Developmental biology 2019 Apr 1;448(1):16-35

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PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial-mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy.

Chang CY, Chen CC, Lin LT, Chang CH, Chen LC, Wang HE, Lee TW, Lee YJ
Cell death discovery 2018;4:100

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Upregulation of LGALS1 is associated with oral cancer metastasis.

Li JM, Tseng CW, Lin CC, Law CH, Chien YA, Kuo WH, Chou HC, Wang WC, Chan HL
Therapeutic advances in medical oncology 2018;10:1758835918794622

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Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Nguyen QH, Pervolarakis N, Blake K, Ma D, Davis RT, James N, Phung AT, Willey E, Kumar R, Jabart E, Driver I, Rock J, Goga A, Khan SA, Lawson DA, Werb Z, Kessenbrock K
Nature communications 2018 May 23;9(1):2028

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Activation of fibroblasts by nicotine promotes the epithelial-mesenchymal transition and motility of breast cancer cells.

Chen PC, Lee WY, Ling HH, Cheng CH, Chen KC, Lin CW
Journal of cellular physiology 2018 Jun;233(6):4972-4980

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The kinesin Eg5 inhibitor K858 induces apoptosis and reverses the malignant invasive phenotype in human glioblastoma cells.

Taglieri L, Rubinacci G, Giuffrida A, Carradori S, Scarpa S
Investigational new drugs 2018 Feb;36(1):28-35

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Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta.

DaSilva-Arnold SC, Zamudio S, Al-Khan A, Alvarez-Perez J, Mannion C, Koenig C, Luke D, Perez AM, Petroff M, Alvarez M, Illsley NP
Biology of reproduction 2018 Aug 1;99(2):409-421

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Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.

Terasaki M, Ogawa Y, Endo T, Maeda H, Hamada J, Osada K, Miyashita K, Mutoh M
Anticancer research 2018 Apr;38(4):2169-2179

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Effects of bradykinin on TGF‑β1‑induced epithelial‑mesenchymal transition in ARPE‑19 cells.

Wei Q, Liu Q, Ren C, Liu J, Cai W, Zhu M, Jin H, He M, Yu J
Molecular medicine reports 2018 Apr;17(4):5878-5886

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Low-Dose Alkylphenol Exposure Promotes Mammary Epithelium Alterations and Transgenerational Developmental Defects, But Does Not Enhance Tumorigenic Behavior of Breast Cancer Cells.

Chamard-Jovenin C, Thiebaut C, Chesnel A, Bresso E, Morel C, Smail-Tabbone M, Devignes MD, Boukhobza T, Dumond H
Frontiers in endocrinology 2017;8:272

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Hypoxia and TGF-β1 induced PLOD2 expression improve the migration and invasion of cervical cancer cells by promoting epithelial-to-mesenchymal transition (EMT) and focal adhesion formation.

Xu F, Zhang J, Hu G, Liu L, Liang W
Cancer cell international 2017;17:54

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Reverse epithelial-mesenchymal transition contributes to the regain of drug sensitivity in tyrosine kinase inhibitor-resistant non-small cell lung cancer cells.

Lee AF, Chen MC, Chen CJ, Yang CJ, Huang MS, Liu YP
PloS one 2017;12(7):e0180383

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Mammary epithelial cell phenotype disruption in vitro and in vivo through ERalpha36 overexpression.

Thiebaut C, Chamard-Jovenin C, Chesnel A, Morel C, Djermoune EH, Boukhobza T, Dumond H
PloS one 2017;12(3):e0173931

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Knockdown of IQGAP1 inhibits proliferation and epithelial-mesenchymal transition by Wnt/β-catenin pathway in thyroid cancer.

Su D, Liu Y, Song T
OncoTargets and therapy 2017;10:1549-1559

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Runx3 is a key modulator during the epithelial-mesenchymal transition of alveolar type II cells in animal models of BPD.

Yang H, Fu J, Yao L, Hou A, Xue X
International journal of molecular medicine 2017 Nov;40(5):1466-1476

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Cytoplasmic aryl hydrocarbon receptor regulates glycogen synthase kinase 3 beta, accelerates vimentin degradation, and suppresses epithelial-mesenchymal transition in non-small cell lung cancer cells.

Li CH, Liu CW, Tsai CH, Peng YJ, Yang YH, Liao PL, Lee CC, Cheng YW, Kang JJ
Archives of toxicology 2017 May;91(5):2165-2178

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Sulfiredoxin may promote metastasis and invasion of cervical squamous cell carcinoma by epithelial-mesenchymal transition.

Chen X, Lan K, Liu Q, Yang X, Wang H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Mar;39(3):1010428317695942

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Downregulation of miR-199a/b-5p is associated with GCNT2 induction upon epithelial-mesenchymal transition in colon cancer.

Chao CC, Wu PH, Huang HC, Chung HY, Chou YC, Cai BH, Kannagi R
FEBS letters 2017 Jul;591(13):1902-1917

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The inhibition of lung cancer cell migration by AhR-regulated autophagy.

Tsai CH, Li CH, Cheng YW, Lee CC, Liao PL, Lin CH, Huang SH, Kang JJ
Scientific reports 2017 Feb 14;7:41927

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Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.

Wang LT, Chiou SS, Chai CY, Hsi E, Chiang CM, Huang SK, Wang SN, Yokoyama KK, Hsu SH
Oncogene 2017 Aug;36(31):4405-4414

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Metronomic Cordycepin Therapy Prolongs Survival of Oral Cancer-Bearing Mice and Inhibits Epithelial-Mesenchymal Transition.

Su NW, Wu SH, Chi CW, Liu CJ, Tsai TH, Chen YJ
Molecules (Basel, Switzerland) 2017 Apr 13;22(4)

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Oxystressed tumor microenvironment potentiates epithelial to mesenchymal transition and alters cellular bioenergetics towards cancer progression.

Sridaran D, Ramamoorthi G, MahaboobKhan R, Kumpati P
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Oct;37(10):13307-13322

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miR-204 inhibits invasion and epithelial-mesenchymal transition by targeting FOXM1 in esophageal cancer.

Sun Y, Yu X, Bai Q
International journal of clinical and experimental pathology 2015;8(10):12775-83

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Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.

Chen CY, Lin YS, Chen CL, Chao PZ, Chiou JF, Kuo CC, Lee FP, Lin YF, Sung YH, Lin YT, Li CF, Chen YJ, Chen CH
Oncotarget 2015 Sep 29;6(29):26946-59

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Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition.

Wang TH, Lin YS, Chen Y, Yeh CT, Huang YL, Hsieh TH, Shieh TM, Hsueh C, Chen TC
Oncotarget 2015 Sep 15;6(27):23342-57

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PGRMC1 contributes to doxorubicin-induced chemoresistance in MES-SA uterine sarcoma.

Lin ST, May EW, Chang JF, Hu RY, Wang LH, Chan HL
Cellular and molecular life sciences : CMLS 2015 Jun;72(12):2395-409

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Comprehensive proteome quantification reveals NgBR as a new regulator for epithelial-mesenchymal transition of breast tumor cells.

Zhao B, Xu B, Hu W, Song C, Wang F, Liu Z, Ye M, Zou H, Miao QR
Journal of proteomics 2015 Jan 1;112:38-52

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Micro-scaffold array chip for upgrading cell-based high-throughput drug testing to 3D using benchtop equipment.

Li X, Zhang X, Zhao S, Wang J, Liu G, Du Y
Lab on a chip 2014 Feb 7;14(3):471-81

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CD63 tetraspanin is a negative driver of epithelial-to-mesenchymal transition in human melanoma cells.

Lupia A, Peppicelli S, Witort E, Bianchini F, Carloni V, Pimpinelli N, Urso C, Borgognoni L, Capaccioli S, Calorini L, Lulli M
The Journal of investigative dermatology 2014 Dec;134(12):2947-2956

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41591

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-cadherin antibody detects E-cadherin protein by Western blot analysis.A. 30 ?g MCF-7 whole cell lysate/extractB. 30 ?g MDA-MB-231 whole cell lysate/extract5 % SDS-PAGEE-cadherin antibody (GTX100443) dilution: 1:2000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-cadherin antibody detects E-cadherin protein by western blot analysis.A. 30 ?g MCF-7 whole cell lysate/extractB. 30 ?g MDA-MB-231 whole cell lysate/extract5 % SDS-PAGEE-cadherin antibody (GTX100443) dilution: 1:2000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-cadherin antibody detects E-cadherin protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-cadherin antibody (GTX100443) diluted at a dilution of 1:2000.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-cadherin antibody detects E-cadherin protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-cadherin antibody (GTX100443) diluted at a dilution of 1:2000.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-cadherin antibody detects E-cadherin protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with E-cadherin antibody (GTX100443) at a dilution of 1:2000.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) MCF-7 whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments.IP samples: MCF-7 whole cell extractA. Control with 3 £gg of preimmune Rabbit IgGB. Immunoprecipitation of E-cadherin protein by 3 £gg E-cadherin antibody (GTX100443)5 % SDS-PAGEThe immunoprecipitated E-cadherin protein was detected by E-cadherin antibody (GTX100443) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 £gg of E-Cadherin antibody (GTX100443).Western blot analysis was performed using E-Cadherin antibody (GTX100443).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. E-cadherin antibody (GTX100443) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. E-Cadherin antibody (GTX100443) diluted at 1:400.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. E-Cadherin antibody (GTX100443) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. E-Cadherin antibody (GTX100443) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. E-Cadherin antibody (GTX100443) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody (GTX100443) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. E-Cadherin antibody (GTX100443) diluted at 1:500.