UM800076

antibody from OriGene

Targeting: CDH1 CD324, UVO, uvomorulin

Provider product page for UM800076

Western blot

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Western blot [2]
Immunohistochemistry [16]

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Product number: UM800076 - Provider product page
Provider: OriGene
Proper citation: OriGene Cat#UM800076, RRID:AB_2629186
Product name: CDH1 mouse monoclonal antibody,clone UMAB184
Antibody type: Monoclonal
Description: CDH1 mouse monoclonal antibody,clone UMAB184
Host: Mouse
Conjugate: Unconjugated
Epitope: CDH1
Isotype: IgG
Antibody clone number: UMAB184
Vial size: 100 µl
Concentration: 1.00mg/ml

CDH1 protein structure - UM800076 shown in red.

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDH1 Mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800076)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800076)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800076)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800076)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human thyroid using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human small intestine using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows mainly membranous staining and weak cytoplasmic staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human skin using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on epithelial cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human prostate cancer using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human placenta using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human ovarian cancer using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human normal cervix using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on squamous epithelial cells of the cervix.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human lung using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human endometrium using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on endometrium.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human endocervical gland using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on epithelial cells of the gland.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: IHC staining of FFPE human breast cancer using anti-CDH1 clone UMAB184 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB. UM800076 requires heat-induced epitope retrieval with Accel pH8.7. The image shows strong membranous and some weak cytoplasmic staining in the tumor cells.
Validation comment: IHC