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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V3232 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- E-Cadherin Antibody / CDH1
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4A2
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the E-Cadherin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Bmi-1 promotes the invasion and migration of colon cancer stem cells through the downregulation of E-cadherin.
The cytoplasmic domain of N-cadherin modulates MMP‑9 induction in oral squamous carcinoma cells.
Clinicopathologic characteristics of 23 cases of invasive low-grade papillary urothelial carcinoma.
RANKL expression specifically observed in vivo promotes epithelial mesenchymal transition and tumor progression.
Slug (SNAI2) expression in oral SCC cells results in altered cell-cell adhesion and increased motility.
Sarcomatoid carcinomas of the lung--are these histogenetically heterogeneous tumors?
Phosphoinositide-3 kinase-Rac1-c-Jun NH2-terminal kinase signaling mediates collagen I-induced cell scattering and up-regulation of N-cadherin expression in mouse mammary epithelial cells.
Apoptosis may underlie the pathology of zinc-deficient skin.
Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn).
Expression of survivin and E-cadherin in breast cancer
E-cadherin phosphorylation by protein kinase D1/protein kinase C{mu} is associated with altered cellular aggregation and motility in prostate cancer.
Gastric medullary carcinoma, a distinct entity associated with microsatellite instability-H, prominent intraepithelial lymphocytes and improved prognosis.
Abnormal expression of molecular markers for bladder impermeability and differentiation in the urothelium of patients with interstitial cystitis.
Acid exposure potentiates intercellular adhesion molecule-1 and e-cadherin expression on A549 alveolar lining epithelial cells.
Independent value of fascin immunoreactivity for predicting lymph node metastases in typical and atypical pulmonary carcinoids.
The role of calpain in the proteolytic cleavage of E-cadherin in prostate and mammary epithelial cells.
E-cadherin expression in pleomorphic lobular carcinoma: an aid to differentiation from ductal carcinoma.
Posttranslational truncation and inactivation of human E-cadherin distinguishes prostate cancer from matched normal prostate.
Clinicopathologic implications of E-cadherin reactivity in patients with lobular carcinoma in situ of the breast.
E-cadherin reactivity of 95 noninvasive ductal and lobular lesions of the breast. Implications for the interpretation of problematic lesions.
N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility.
Mechanism of extracellular domain-deleted dominant negative cadherins.
Zhang Z, Bu X, Chen H, Wang Q, Sha W
International journal of molecular medicine 2016 Oct;38(4):1199-207
International journal of molecular medicine 2016 Oct;38(4):1199-207
The cytoplasmic domain of N-cadherin modulates MMP‑9 induction in oral squamous carcinoma cells.
Walker A, Frei R, Lawson KR
International journal of oncology 2014 Oct;45(4):1699-706
International journal of oncology 2014 Oct;45(4):1699-706
Clinicopathologic characteristics of 23 cases of invasive low-grade papillary urothelial carcinoma.
Watts KE, Montironi R, Mazzucchelli R, van der Kwast T, Osunkoya AO, Stephenson AJ, Hansel DE
Urology 2012 Aug;80(2):361-6
Urology 2012 Aug;80(2):361-6
RANKL expression specifically observed in vivo promotes epithelial mesenchymal transition and tumor progression.
Yamada T, Tsuda M, Takahashi T, Totsuka Y, Shindoh M, Ohba Y
The American journal of pathology 2011 Jun;178(6):2845-56
The American journal of pathology 2011 Jun;178(6):2845-56
Slug (SNAI2) expression in oral SCC cells results in altered cell-cell adhesion and increased motility.
Katafiasz D, Smith LM, Wahl JK 3rd
Cell adhesion & migration 2011 Jul-Aug;5(4):315-22
Cell adhesion & migration 2011 Jul-Aug;5(4):315-22
Sarcomatoid carcinomas of the lung--are these histogenetically heterogeneous tumors?
Blaukovitsch M, Halbwedl I, Kothmaier H, Gogg-Kammerer M, Popper HH
Virchows Archiv : an international journal of pathology 2006 Oct;449(4):455-61
Virchows Archiv : an international journal of pathology 2006 Oct;449(4):455-61
Phosphoinositide-3 kinase-Rac1-c-Jun NH2-terminal kinase signaling mediates collagen I-induced cell scattering and up-regulation of N-cadherin expression in mouse mammary epithelial cells.
Shintani Y, Wheelock MJ, Johnson KR
Molecular biology of the cell 2006 Jul;17(7):2963-75
Molecular biology of the cell 2006 Jul;17(7):2963-75
Apoptosis may underlie the pathology of zinc-deficient skin.
Wilson D, Varigos G, Ackland ML
Immunology and cell biology 2006 Feb;84(1):28-37
Immunology and cell biology 2006 Feb;84(1):28-37
Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn).
Maeda M, Johnson E, Mandal SH, Lawson KR, Keim SA, Svoboda RA, Caplan S, Wahl JK 3rd, Wheelock MJ, Johnson KR
Oncogene 2006 Aug 3;25(33):4595-604
Oncogene 2006 Aug 3;25(33):4595-604
Expression of survivin and E-cadherin in breast cancer
Tian X, Liu J, Wang L, Feng X, Yao J
Chinese Journal of Cancer Research 2005 June;17(2):103-106
Chinese Journal of Cancer Research 2005 June;17(2):103-106
E-cadherin phosphorylation by protein kinase D1/protein kinase C{mu} is associated with altered cellular aggregation and motility in prostate cancer.
Jaggi M, Rao PS, Smith DJ, Wheelock MJ, Johnson KR, Hemstreet GP, Balaji KC
Cancer research 2005 Jan 15;65(2):483-92
Cancer research 2005 Jan 15;65(2):483-92
Gastric medullary carcinoma, a distinct entity associated with microsatellite instability-H, prominent intraepithelial lymphocytes and improved prognosis.
Lü BJ, Lai M, Cheng L, Xu JY, Huang Q
Histopathology 2004 Nov;45(5):485-92
Histopathology 2004 Nov;45(5):485-92
Abnormal expression of molecular markers for bladder impermeability and differentiation in the urothelium of patients with interstitial cystitis.
Slobodov G, Feloney M, Gran C, Kyker KD, Hurst RE, Culkin DJ
The Journal of urology 2004 Apr;171(4):1554-8
The Journal of urology 2004 Apr;171(4):1554-8
Acid exposure potentiates intercellular adhesion molecule-1 and e-cadherin expression on A549 alveolar lining epithelial cells.
Ohwada A, Tsutsumi-Ishii Y, Yoshioka Y, Iwabuchi K, Nagaoka I, Fukuchi Y
Experimental lung research 2003 Sep;29(6):389-400
Experimental lung research 2003 Sep;29(6):389-400
Independent value of fascin immunoreactivity for predicting lymph node metastases in typical and atypical pulmonary carcinoids.
Pelosi G, Pasini F, Fraggetta F, Pastorino U, Iannucci A, Maisonneuve P, Arrigoni G, De Manzoni G, Bresaola E, Viale G
Lung cancer (Amsterdam, Netherlands) 2003 Nov;42(2):203-13
Lung cancer (Amsterdam, Netherlands) 2003 Nov;42(2):203-13
The role of calpain in the proteolytic cleavage of E-cadherin in prostate and mammary epithelial cells.
Rios-Doria J, Day KC, Kuefer R, Rashid MG, Chinnaiyan AM, Rubin MA, Day ML
The Journal of biological chemistry 2003 Jan 10;278(2):1372-9
The Journal of biological chemistry 2003 Jan 10;278(2):1372-9
E-cadherin expression in pleomorphic lobular carcinoma: an aid to differentiation from ductal carcinoma.
Wahed A, Connelly J, Reese T
Annals of diagnostic pathology 2002 Dec;6(6):349-51
Annals of diagnostic pathology 2002 Dec;6(6):349-51
Posttranslational truncation and inactivation of human E-cadherin distinguishes prostate cancer from matched normal prostate.
Rashid MG, Sanda MG, Vallorosi CJ, Rios-Doria J, Rubin MA, Day ML
Cancer research 2001 Jan 15;61(2):489-92
Cancer research 2001 Jan 15;61(2):489-92
Clinicopathologic implications of E-cadherin reactivity in patients with lobular carcinoma in situ of the breast.
Goldstein NS, Kestin LL, Vicini FA
Cancer 2001 Aug 15;92(4):738-47
Cancer 2001 Aug 15;92(4):738-47
E-cadherin reactivity of 95 noninvasive ductal and lobular lesions of the breast. Implications for the interpretation of problematic lesions.
Goldstein NS, Bassi D, Watts JC, Layfield LJ, Yaziji H, Gown AM
American journal of clinical pathology 2001 Apr;115(4):534-42
American journal of clinical pathology 2001 Apr;115(4):534-42
N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility.
Kim JB, Islam S, Kim YJ, Prudoff RS, Sass KM, Wheelock MJ, Johnson KR
The Journal of cell biology 2000 Dec 11;151(6):1193-206
The Journal of cell biology 2000 Dec 11;151(6):1193-206
Mechanism of extracellular domain-deleted dominant negative cadherins.
Nieman MT, Kim JB, Johnson KR, Wheelock MJ
Journal of cell science 1999 May;112 ( Pt 10):1621-32
Journal of cell science 1999 May;112 ( Pt 10):1621-32
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human skin with E-Cadherin antibody (clone 4A2). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free E-Cadherin Antibody (clone 4A2). Confirmation of Integrity and Purity of the Antibody.
