Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- AM20867PU-N - Provider product page
- Provider
- OriGene
- Product name
- E Cadherin (CDH1) mouse monoclonal antibody, clone 3F4, Purified
- Antibody type
- Monoclonal
- Description
- E Cadherin (CDH1) mouse monoclonal antibody, clone 3F4, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CDH1
- Isotype
- IgG
- Antibody clone number
- 3F4
- Vial size
- 50 µg
- Concentration
- 0.4 mg/ml
Submitted references Downregulation of p53 drives autophagy during human trophoblast differentiation.
Gauster M, Maninger S, Siwetz M, Deutsch A, El-Heliebi A, Kolb-Lenz D, Hiden U, Desoye G, Herse F, Prokesch A
Cellular and molecular life sciences : CMLS 2018 May;75(10):1839-1855
Cellular and molecular life sciences : CMLS 2018 May;75(10):1839-1855
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of CDH1 expression in transfected 293T cell line by CDH1 monoclonal antibody, clone 3F4.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis (PLA) of protein-protein interactions between EGFR and CDH1 HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1/1200 and anti-CDH1 mouse monoclonal antibody 1/50.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CDH1 on formalin-fixed paraffin-embedded human stomach (antibody concentration 3 ug/ml). Formalin-Fixed, Paraffin-Embedded Human Stomach stained with CD324 / Cadherin-1 Antibody (Clone 3F4) at 3 ug/ml.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded Human Spleen stained with CD324 / Cadherin-1 Antibody (Clone 3F4) at 3 ug/ml after heat-induced antigen retrieval.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded Human Lung stained with CD324 / Cadherin-1 Antibody (Clone 3F4) at 3 ug/ml after heat-induced antigen retrieval.
- Validation comment
- IHC