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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- PAB3139 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3139, RRID:AB_1571932
- Product name
- CDH1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CDH1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references KCl cotransporter-3 down-regulates E-cadherin/beta-catenin complex to promote epithelial-mesenchymal transition.
Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin.
Characterization and chromosomal localization of the gene encoding the human cell adhesion molecule uvomorulin.
Hsu YM, Chen YF, Chou CY, Tang MJ, Chen JH, Wilkins RJ, Ellory JC, Shen MR
Cancer research 2007 Nov 15;67(22):11064-73
Cancer research 2007 Nov 15;67(22):11064-73
Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin.
Knudsen KA, Wheelock MJ
The Journal of cell biology 1992 Aug;118(3):671-9
The Journal of cell biology 1992 Aug;118(3):671-9
Characterization and chromosomal localization of the gene encoding the human cell adhesion molecule uvomorulin.
Mansouri A, Spurr N, Goodfellow PN, Kemler R
Differentiation; research in biological diversity 1988 Jun;38(1):67-71
Differentiation; research in biological diversity 1988 Jun;38(1):67-71
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CDH1 (arrow) using rabbit CDH1 polyclonal antibody (Cat # PAB3139). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH1 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CDH1 polyclonal antibody (Cat # PAB3139) in 293 cell line lysates (35 ug/lane). CDH1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H292 cells using CDH1 polyclonal antibody (Cat # PAB3139)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
