LS-B16725

antibody from LSBio

Targeting: CDH1 CD324, UVO, uvomorulin

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B16725

Provider

LSBio

Product name

IHC-plus™ CDH1 / E Cadherin Antibody LS-B16725

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: 293 whole cell lysate All Lanes: CDH1 antibody at 2.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 98, 91 KDa Observed band size: 125 KDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating CDH1 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CDH1 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CDH1 Antibody (6µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg)