- MA5-16086 - Invitrogen Antibodies TNC antibody

Submitted references The Effects of Cold Water Immersion and Active Recovery on Molecular Factors That Regulate Growth and Remodeling of Skeletal Muscle After Resistance Exercise.

Peake JM, Markworth JF, Cumming KT, Aas SN, Roberts LA, Raastad T, Cameron-Smith D, Figueiredo VC
Frontiers in physiology 2020;11:737

Targeted disruption of the iNOS gene improves adipose tissue inflammation and fibrosis in leptin-deficient ob/ob mice: role of tenascin C.

Becerril S, Rodríguez A, Catalán V, Méndez-Giménez L, Ramírez B, Sáinz N, Llorente M, Unamuno X, Gómez-Ambrosi J, Frühbeck G
International journal of obesity (2005) 2018 Aug;42(8):1458-1470

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Tenascin C in formalin fixed paraffin embedded tissue section of mouse bone-tendon. Samples were incubated in Tenascin C monoclonal antibody (Product # MA5-16086) using a dilution of 1:25. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Tenascin C in formalin fixed paraffin embedded tissue section of mouse bone-tendon. Samples were incubated in Tenascin C monoclonal antibody (Product # MA5-16086) using a dilution of 1:25. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Tenascin C in U87MG Cells. Samples were incubated in Tenascin C monoclonal antibody (Product # MA5-16086) and a matched isotype control using a dilution of 1 µg/mL for 30 minutes at room temperature followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody. Figure A: Antibody (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Figure B: Untreated cells (orange) or treated cells with 3 µM Monensin (blue). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Tenascin C in SK-MEL-28 cells. Samples were incubated in Tenascin C monoclonal antibody (Product # MA5-16086) and a matched isotype control using a dilution of 2.5 µg/mL for 30 minutes at room temperature followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Tenascin C in SK-MEL-28 cells (blue) and a matched isotype control (orange). Samples were incubated in Tenascin C monoclonal antibody (Product # MA5-16086) using a dilution of 5 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of Tenascin C in 1 x 10^6 MCF-7 cells. Samples were incubated in Tenascin C monoclonal antibody (Product # MA5-16086) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.

MA5-16086

Antibody data