AF947

antibody from R&D Systems

Targeting: DSG2 CDHF5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF947

Provider

R&D Systems

Product name

Human Desmoglein-2 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Desmoglein-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Desmoglein-1 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

CAA81226


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Penton-dodecahedral particles trigger opening of intercellular junctions and facilitate viral spread during adenovirus serotype 3 infection of epithelial cells.

Lu ZZ, Wang H, Zhang Y, Cao H, Li Z, Fender P, Lieber A
PLoS pathogens 2013 Oct;9(10):e1003718

---

Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells.

van Rensburg R, Beyer I, Yao XY, Wang H, Denisenko O, Li ZY, Russell DW, Miller DG, Gregory P, Holmes M, Bomsztyk K, Lieber A
Gene therapy 2013 Feb;20(2):201-14

---

A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses.

Wang H, Beyer I, Persson J, Song H, Li Z, Richter M, Cao H, van Rensburg R, Yao X, Hudkins K, Yumul R, Zhang XB, Yu M, Fender P, Hemminki A, Lieber A
Journal of virology 2012 Jun;86(11):6286-302

---

Multimerization of adenovirus serotype 3 fiber knob domains is required for efficient binding of virus to desmoglein 2 and subsequent opening of epithelial junctions.

Wang H, Li Z, Yumul R, Lara S, Hemminki A, Fender P, Lieber A
Journal of virology 2011 Jul;85(13):6390-402

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Desmoglein-2 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Desmoglein-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF947) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Desmoglein-2 at approximately 90-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Desmoglein-2 by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Desmoglein-2 at approximately 159-164 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Desmoglein-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF947) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.