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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- MAB947 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Desmoglein-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Desmoglein-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25-50% cross-reactivity with recombinant human (rh) Desmoglein-1 is observed and no cross-reactivity with rhDesmoglein-3 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
CAA81226- Isotype
- IgG
- Antibody clone number
- 141409
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Prospective isolation and global gene expression analysis of definitive and visceral endoderm.
Sherwood RI, Jitianu C, Cleaver O, Shaywitz DA, Lamenzo JO, Chen AE, Golub TR, Melton DA
Developmental biology 2007 Apr 15;304(2):541-55
Developmental biology 2007 Apr 15;304(2):541-55
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Desmoglein-2 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoglein-2 Monoclonal Antibody (Catalog # MAB947) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Desmoglein-2 at approximately 90-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Desmoglein-2 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Desmoglein-2 at approximately 120 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Desmoglein-2 Monoclonal Antibody (Catalog # MAB947). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Desmoglein-2 in NHEK Human Cells. Desmoglein-2 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Desmoglein-2 Monoclonal Antibody (Catalog # MAB947) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Desmoglein-2 in A431 Human Cell Line. Desmoglein-2 was detected in immersion fixed A431 human epithelial carcinoma cell line wildtype and knockout using Mouse Anti-Human Desmoglein-2 Monoclonal Antibody (Catalog # MAB947) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
