LS-C800192

antibody from LSBio

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

ELISA

Immunocytochemistry

Antibody data

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Validation data

Product number: LS-C800192 - Provider product page
Provider: LSBio
Product name: AKT1 Antibody (phospho-Ser473) LS-C800192
Antibody type: Polyclonal
Description: Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Storage: Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

AKT1 protein structure - LS-C800192 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Phospho-Akt(Ser473) antibody for IHC in human brain tissue

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Phospho-Akt(Ser473) antibody for IHC in human brain tissue

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: 1:200 staining human lung carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.