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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- A301-004A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BIG2/ARFGEF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BIG2/ARFGEF2 by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 antibody A301-004A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BIG2/AFGEF2 was also immunoprecipitated by rabbit anti-BIG2/ARFGEF2 antibody BL4399, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BIG2/ARFGEF2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG2/ARFGEF2 (Cat. No. A301-004A) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
