Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4744 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TBX6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TBX6 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Generation of eight human induced pluripotent stem cell lines from Parkinson's disease patients carrying familial mutations.
Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility.
Chen M, Maimaitili M, Buchholdt SH, Jensen UB, Febbraro F, Denham M
Stem cell research 2020 Jan;42:101657
Stem cell research 2020 Jan;42:101657
Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
Garriock RJ, Chalamalasetty RB, Kennedy MW, Canizales LC, Lewandoski M, Yamaguchi TP
Development (Cambridge, England) 2015 May 1;142(9):1628-38
Development (Cambridge, England) 2015 May 1;142(9):1628-38
In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
Gouti M, Tsakiridis A, Wymeersch FJ, Huang Y, Kleinjung J, Wilson V, Briscoe J
PLoS biology 2014 Aug;12(8):e1001937
PLoS biology 2014 Aug;12(8):e1001937
Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility.
Cheung C, Bernardo AS, Trotter MW, Pedersen RA, Sinha S
Nature biotechnology 2012 Jan 15;30(2):165-73
Nature biotechnology 2012 Jan 15;30(2):165-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TBX6 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TBX6 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TBX6 by Western Blot. Detection of Human TBX6 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- TBX6 in Embryonic Mouse Mesoderm. TBX6 in Embryonic Mouse Mesoderm.