PAB7027

antibody from Abnova Corporation

Targeting: NONO NMT55, NRB54, P54, P54NRB, PPP1R114

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB7027

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB7027, RRID:AB_1578110

Product name

NONO polyclonal antibody

Antibody type

Polyclonal

Description

Goat polyclonal antibody raised against synthetic peptide of NONO.

Antigen sequence

C-NRAAPGAEFAPNK


Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

NONO and RALY proteins are required for YB-1 oxaliplatin induced resistance in colon adenocarcinoma cell lines.

Tsofack SP, Garand C, Sereduk C, Chow D, Aziz M, Guay D, Yin HH, Lebel M
Molecular cancer 2011 Nov 25;10:145

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PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator.

Brown SA, Ripperger J, Kadener S, Fleury-Olela F, Vilbois F, Rosbash M, Schibler U
Science (New York, N.Y.) 2005 Apr 29;308(5722):693-6

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NONO polyclonal antibody (Cat # PAB7027) (0.1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

HEK293 overexpressing human NONO and probed with NONO polyclonal antibody (Cat # PAB7027) (mock transfection in first lane), tested by Origene.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NONO polyclonal antibody (Cat # PAB7027)(3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)