18148-1-AP

antibody from Invitrogen Antibodies

Targeting: UBE4B E4, KIAA0684, UBOX3, UFD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18148-1-AP

Provider

Invitrogen Antibodies

Product name

UBE4B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EMIENHPLS TKLLVPSLMK FYTDVEHTGA TSEFYDKFTI RYHISTIFKS LWQNIAHHGT FMEEFNSGKQ FVRYINMLIN DTTFLLDESL ESLKRIHEVQ EEMKNKEQWD QLPRDQQQAR QSQLAQDERV SRSYLALATE TVDMFHILTK QVQKPFLRPE LGPRLAAMLN FNLQQLCGPK CRDLKVENPE KYGFEPKKLL DQLTDIYLQL DCARFAKAIA DDQRSYSKEL FEEVISKMRK AGIKSTIAIE KFKLLAEKVE EIVAKNARAE IDYSDAPDEF RDPLMDTLMT DPVRLPSGTI MDRSIILRHL LNSPTDPFNR QTLTESMLEP VPELKEQIQA WMREKQNSDH (825-1173 aa encoded by BC093696)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 18148-1-AP (UBE4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18148-1-AP ( UBE4B Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18148-1-AP (UBE4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18148-1-AP ( UBE4B Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 18148-1-AP (UBE4B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 18148-1-AP (UBE4B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 18148-1-AP ( UBE4B Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 18148-1-AP ( UBE4B Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-UBE4B (IP:18148-1-AP, 4ug; Detection:18148-1-AP 1:2000) with HeLa cells lysate 1200ug.