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Learn14551-1-AP150UL
antibody from Invitrogen Antibodies
Targeting: NACC1
BEND8, BTBD14B, BTBD30, NAC-1, NAC1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- 14551-1-AP150UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NACC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates (LNCaP, MCF-7) were subjected to SDS PAGE followed by western blot with (Product # 14551-1-AP150UL) (NACC1 antibody) at dilution of 1:3,000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with (Product # 14551-1-AP150UL) (NACC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using NACC1 antibody (Product # 14551-1-AP150UL)) at dilution of 1:400 and CoraLite 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using (Product # 14551-1-AP150UL) (NACC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using (Product # 14551-1-AP150UL) (NACC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue slide using (Product # 14551-1-AP150UL) (NACC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue slide using (Product # 14551-1-AP150UL) (NACC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue slide using (Product # 14551-1-AP150UL) (NACC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP Result of anti-NACC1 (IP:(Product # 14551-1-AP150UL), 4 µg; Detection:(Product # 14551-1-AP150UL) 1:300) with MCF-7 cells lysate 3200 µg.
