PAB21429

antibody from Abnova Corporation

Targeting: SMCR8 DENND8A, FLJ34716

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21429

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21429, RRID:AB_10967375

Product name

SMCR8 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant SMCR8.

Antigen sequence

SQASTTSNPDESADTDLYTCRPAYTPKLIKAKSTK
CFDKKLKTLEELCDTEYFTQTLAQLSHIEHMFRGD
LCYLLTSQIDRALLKQQHITNFLFEDFVEVDDRMV
EKQESIPSKPSQD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with SMCR8 polyclonal antibody (Cat # PAB21429) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows positivity in nuclei but not nucleoli.Using SMCR8 polyclonal antibody (Cat # PAB21429).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with SMCR8 polyclonal antibody (Cat # PAB21429) shows strong nuclear positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)