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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-38273 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMCR8 Recombinant Rabbit Monoclonal Antibody (HL1007)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Centrifuge briefly prior to opening.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL1007
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMCR8 in Various tissue extracts (50 µg). Samples were separated with 5% SDS-PAGE and incubated in SMCR8 monoclonal antibody (Product # MA5-38273) using a dilution of 1:1,000, followed by HRP-conjugated anti-rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMCR8 in Various whole cell extracts (30 µg). Samples were separated with 5% SDS-PAGE and incubated in SMCR8 monoclonal antibody (Product # MA5-38273) using a dilution of 1:1,000, followed by HRP-conjugated anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMCR8 Recombinant Rabbit Monoclonal Antibody (HL1007) detects SMCR8 protein at nucleus by immunofluorescent analysis. Sample: DIV10 rat E19 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMCR8 stained by SMCR8 Recombinant Rabbit Monoclonal Antibody (HL1007) (Product # MA5-38273) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SMCR8 in paraffin-embedded mouse intestine. Samples was treated with Citrate buffer (pH 6, 15 min) for antigen retrieval and incubated in SMCR8 monoclonal antibody (Product # MA5-38273) using a dilution of 1:100.
