Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-117431 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMCR8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Centrifuge briefly prior to opening.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.12 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMCR8 in Various whole cell extracts (30 µg). Samples were separated with 5% SDS-PAGE and incubated in SMCR8 polyclonal antibody (Product # PA5-117431) using a dilution of 1:500, followed by HRP-conjugated anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SMCR8 in 4% paraformaldehyde (RT, 15 min) fixed DIV10 rat E18 primary cortical neuron cells (Green: SMCR8, Red: Tau). Samples was incubated in SMCR8 polyclonal antibody (Product # PA5-117431) using a dilution of 1:500, followed by DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMCR8 Polyclonal Antibody detects SMCR8 protein at nucleus by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMCR8 stained by SMCR8 Polyclonal Antibody (Product # PA5-117431) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMCR8 Polyclonal Antibody detects SMCR8 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SMCR8 stained by SMCR8 Polyclonal Antibody (Product # PA5-117431) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.