16245-1-AP

antibody from Invitrogen Antibodies

Targeting: TBRG4 Cpr2, FASTKD4, H_TD2522F11.8, KIAA0948

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16245-1-AP

Provider

Invitrogen Antibodies

Product name

TBRG4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SYHLVQKPF SLTKDVLLDV AYAYGKLSFH QTQVSQRLAT DLLSLMPSLT SGEVAHCAKS FALLKWLSLP LFEAFAQHVL NRAQDITLPH LCSVLLAFAR LNFHPDQEDQ FFSLVHEKLG SELPGLEPAL QVDLVWALCV LQQAREAELQ AVLHPEFHIQ FLGGKSQKDQ NTFQKLLHIN ATALLEYPEY SGPLLPASAV APGPSALDRK VTPLQKELQE TLKGLLGSAD KGSLEVATQY GWVLDAEVLL DSDGEFLPVR DFVAPHLAQP TGSQSPPPGS KRLAFLRWEF PNFNSRSKDL LGRFVLARRH IVAAGFLIVD VPFYEWLELK SEWQKGAYLK DKMRKAVAEE LAK (280-631 aa encoded by BC017235)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result of 16245-1-AP (anti-KD4) in COS7 cell by Dr. Nancy Kedersha. COS7 cells, fixed with 4% paraformaldehyde, post/fied and permeabilized with methanol, and stained with PTG anti-FAST KD4 antibody (16245-1-AP) in green, and human anti-mito autoimune sera from Immunovision in red. Nuclei are stained blue using Hoechst dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using TBRG4 antibody 16245-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result of anti-TBRG4 (16245-1-AP) with U2OS cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16245-1-AP ( TBRG4 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16245-1-AP ( TBRG4 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TBRG4 (IP:16245-1-AP, 4ug; Detection:16245-1-AP 1:500) with HepG2 cells lysate 1600ug.