Antibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 16215-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16215-1-AP, RRID:AB_2220210
- Product name
- ACAT1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ACAT1 antibody (Cat. #16215-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.
Dietary iron overload mitigates atherosclerosis in high-fat diet-fed apolipoprotein E knockout mice: Role of dysregulated hepatic fatty acid metabolism.
Improvement of hyperlipidemia by aerobic exercise in mice through a regulatory effect of miR-21a-5p on its target genes.
TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.
Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1.
ACAT1 and Metabolism-Related Pathways Are Essential for the Progression of Clear Cell Renal Cell Carcinoma (ccRCC), as Determined by Co-expression Network Analysis.
Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.
TGIF transcription factors repress acetyl CoA metabolic gene expression and promote intestinal tumor growth.
Dysregulation of Neuronal Cholesterol Homeostasis upon Exposure to HIV-1 Tat and Cocaine Revealed by RNA-Sequencing.
Nutritional Ketosis Affects Metabolism and Behavior in Sprague-Dawley Rats in Both Control and Chronic Stress Environments.
Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.
Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages.
Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells.
Partial complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage.
2-Deoxy-D-glucose treatment induces ketogenesis, sustains mitochondrial function, and reduces pathology in female mouse model of Alzheimer's disease.
Zhang L, Zhang Z, Li C, Zhu T, Gao J, Zhou H, Zheng Y, Chang Q, Wang M, Wu J, Ran L, Wu Y, Miao H, Zou X, Liang B
Cellular and molecular gastroenterology and hepatology 2021;11(3):697-724
Cellular and molecular gastroenterology and hepatology 2021;11(3):697-724
Dietary iron overload mitigates atherosclerosis in high-fat diet-fed apolipoprotein E knockout mice: Role of dysregulated hepatic fatty acid metabolism.
Xiao L, Luo G, Li H, Yao P, Tang Y
Biochimica et biophysica acta. Molecular and cell biology of lipids 2021 Oct;1866(10):159004
Biochimica et biophysica acta. Molecular and cell biology of lipids 2021 Oct;1866(10):159004
Improvement of hyperlipidemia by aerobic exercise in mice through a regulatory effect of miR-21a-5p on its target genes.
Zhao J, Song Y, Zeng Y, Chen L, Yan F, Chen A, Wu B, Wang Y
Scientific reports 2021 Jun 7;11(1):11966
Scientific reports 2021 Jun 7;11(1):11966
TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.
Huang D, Xu B, Liu L, Wu L, Zhu Y, Ghanbarpour A, Wang Y, Chen FJ, Lyu J, Hu Y, Kang Y, Zhou W, Wang X, Ding W, Li X, Jiang Z, Chen J, Zhang X, Zhou H, Li JZ, Guo C, Zheng W, Zhang X, Li P, Melia T, Reinisch K, Chen XW
Cell metabolism 2021 Aug 3;33(8):1655-1670.e8
Cell metabolism 2021 Aug 3;33(8):1655-1670.e8
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
Li ST, Huang, Shen S, Cai Y, Xing S, Wu G, Jiang Z, Hao Y, Yuan M, Wang N, Zhu L, Yan R, Yang D, Wang L, Liu Z, Hu X, Zhou R, Qu K, Li A, Duan X, Zhang H, Gao P
Nature metabolism 2020 Mar;2(3):256-269
Nature metabolism 2020 Mar;2(3):256-269
Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.
Ceccacci S, Deitersen J, Mozzicafreddo M, Morretta E, Proksch P, Wesselborg S, Stork B, Monti MC
Biomolecules 2020 Jun 2;10(6)
Biomolecules 2020 Jun 2;10(6)
Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1.
Guan J, Jiang X, Gai J, Sun X, Zhao J, Li J, Li Y, Cheng M, Du T, Fu L, Li Q
Journal of cellular and molecular medicine 2020 Dec;24(23):14039-14049
Journal of cellular and molecular medicine 2020 Dec;24(23):14039-14049
ACAT1 and Metabolism-Related Pathways Are Essential for the Progression of Clear Cell Renal Cell Carcinoma (ccRCC), as Determined by Co-expression Network Analysis.
Chen L, Peng T, Luo Y, Zhou F, Wang G, Qian K, Xiao Y, Wang X
Frontiers in oncology 2019;9:957
Frontiers in oncology 2019;9:957
Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.
Zhong S, Li L, Zhang YL, Zhang L, Lu J, Guo S, Liang N, Ge J, Zhu M, Tao Y, Wu YC, Yin H
The Journal of clinical investigation 2019 Jan 2;129(1):252-267
The Journal of clinical investigation 2019 Jan 2;129(1):252-267
TGIF transcription factors repress acetyl CoA metabolic gene expression and promote intestinal tumor growth.
Shah A, Melhuish TA, Fox TE, Frierson HF Jr, Wotton D
Genes & development 2019 Apr 1;33(7-8):388-402
Genes & development 2019 Apr 1;33(7-8):388-402
Dysregulation of Neuronal Cholesterol Homeostasis upon Exposure to HIV-1 Tat and Cocaine Revealed by RNA-Sequencing.
Mohseni Ahooyi T, Shekarabi M, Torkzaban B, Langford TD, Burdo TH, Gordon J, Datta PK, Amini S, Khalili K
Scientific reports 2018 Nov 2;8(1):16300
Scientific reports 2018 Nov 2;8(1):16300
Nutritional Ketosis Affects Metabolism and Behavior in Sprague-Dawley Rats in Both Control and Chronic Stress Environments.
Brownlow ML, Jung SH, Moore RJ, Bechmann N, Jankord R
Frontiers in molecular neuroscience 2017;10:129
Frontiers in molecular neuroscience 2017;10:129
Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.
Huang, Li T, Wang L, Zhang L, Yan R, Li K, Xing S, Wu G, Hu L, Jia W, Lin SC, Dang CV, Song L, Gao P, Zhang H
Cell research 2016 Oct;26(10):1112-1130
Cell research 2016 Oct;26(10):1112-1130
Effects of Porphyromonas gingivalis lipopolysaccharide on the expression of key genes involved in cholesterol metabolism in macrophages.
Liu F, Wang Y, Xu J, Liu F, Hu R, Deng H
Archives of medical science : AMS 2016 Oct 1;12(5):959-967
Archives of medical science : AMS 2016 Oct 1;12(5):959-967
Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells.
Hao S, Ji J, Zhao H, Shang L, Wu J, Li H, Qiao T, Li K
Molecules (Basel, Switzerland) 2015 Dec 1;20(12):21287-97
Molecules (Basel, Switzerland) 2015 Dec 1;20(12):21287-97
Partial complex I deficiency due to the CNS conditional ablation of Ndufa5 results in a mild chronic encephalopathy but no increase in oxidative damage.
Peralta S, Torraco A, Wenz T, Garcia S, Diaz F, Moraes CT
Human molecular genetics 2014 Mar 15;23(6):1399-412
Human molecular genetics 2014 Mar 15;23(6):1399-412
2-Deoxy-D-glucose treatment induces ketogenesis, sustains mitochondrial function, and reduces pathology in female mouse model of Alzheimer's disease.
Yao J, Chen S, Mao Z, Cadenas E, Brinton RD
PloS one 2011;6(7):e21788
PloS one 2011;6(7):e21788
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16215-1-AP(ACAT1 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using ACAT1 antibody 16215-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 16215-1-AP(ACAT1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 16215-1-AP(ACAT1 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue