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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [3]
- Immunohistochemistry [2]
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- Product number
- TA505437 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA505437, RRID:AB_2623090
- Product name
- GADD45G mouse monoclonal antibody, clone OTI2F12 (formerly 2F12)
- Antibody type
- Monoclonal
- Description
- GADD45G mouse monoclonal antibody, clone OTI2F12 (formerly 2F12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GADD45G
- Isotype
- IgG
- Antibody clone number
- OTI2F12
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references GADD45γ induces G2/M arrest in human pharynx and nasopharyngeal carcinoma cells by cucurbitacin E.
Inducement of mitosis delay by cucurbitacin E, a novel tetracyclic triterpene from climbing stem of Cucumis melo L., through GADD45γ in human brain malignant glioma (GBM) 8401 cells.
Therapeutic ROS targeting of GADD45γ in the induction of G2/M arrest in primary human colorectal cancer cell lines by cucurbitacin E.
Hung CM, Chang CC, Lin CW, Chen CC, Hsu YC
Scientific reports 2014 Sep 23;4:6454
Scientific reports 2014 Sep 23;4:6454
Inducement of mitosis delay by cucurbitacin E, a novel tetracyclic triterpene from climbing stem of Cucumis melo L., through GADD45γ in human brain malignant glioma (GBM) 8401 cells.
Hsu YC, Chen MJ, Huang TY
Cell death & disease 2014 Feb 27;5(2):e1087
Cell death & disease 2014 Feb 27;5(2):e1087
Therapeutic ROS targeting of GADD45γ in the induction of G2/M arrest in primary human colorectal cancer cell lines by cucurbitacin E.
Hsu YC, Huang TY, Chen MJ
Cell death & disease 2014 Apr 24;5(4):e1198
Cell death & disease 2014 Apr 24;5(4):e1198
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G (RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GADD45G mouse monoclonal antibody (TA505437) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G(RC201364).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human NPC cell lines.
- Validation comment
- IP
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human GBM8401 cells.
- Validation comment
- IP
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunoprecipitation of GADD45G protein level by using anti-GADD45G antibody in human CRC cells.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505437)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GADD45G mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505437)
- Validation comment
- IHC
