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- Antibody Data
- Antigen structure
- References [30]
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- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF470 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCL13/BLC/BCA-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse CXCL13/BLC/BCA-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q3U1E8- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-4Rα-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells.
The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity.
Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp.
Human Adipose Tissue-Derived Mesenchymal Stem Cells Attenuate Atopic Dermatitis by Regulating the Expression of MIP-2, miR-122a-SOCS1 Axis, and Th1/Th2 Responses.
Inhaled TLR9 Agonist Renders Lung Tumors Permissive to PD-1 Blockade by Promoting Optimal CD4+ and CD8+ T-cell Interplay.
Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice.
Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection.
Hyperactive gp130/STAT3-driven gastric tumourigenesis promotes submucosal tertiary lymphoid structure development.
Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity.
Ageing adversely affects the migration and function of marginal zone B cells.
Role of SIRPα in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen.
Brain inflammation is accompanied by peripheral inflammation in Cstb -/- mice, a model for progressive myoclonus epilepsy.
Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia.
Stromal cells as trend-setters for cells migrating into the lymph node.
Anti-CXCL13 antibody can inhibit the formation of gastric lymphoid follicles induced by Helicobacter infection.
CLEC-2 is required for development and maintenance of lymph nodes.
Repetitive hypoxic preconditioning induces an immunosuppressed B cell phenotype during endogenous protection from stroke.
IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles.
CXCL13 is elevated in Sjögren's syndrome in mice and humans and is implicated in disease pathogenesis.
IL-23 is required for long-term control of Mycobacterium tuberculosis and B cell follicle formation in the infected lung.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines.
Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice.
Role of lymphotoxin-alpha in cigarette smoke-induced inflammation and lymphoid neogenesis.
Suppression of established experimental autoimmune encephalomyelitis and formation of meningeal lymphoid follicles by lymphotoxin beta receptor-Ig fusion protein.
Regulation of B1 cell migration by signals through Toll-like receptors.
The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
Dubey LK, Ludewig B, Luther SA, Harris NL
Cell reports 2019 May 21;27(8):2442-2458.e5
Cell reports 2019 May 21;27(8):2442-2458.e5
The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity.
Billi AC, Gharaee-Kermani M, Fullmer J, Tsoi LC, Hill BD, Gruszka D, Ludwig J, Xing X, Estadt S, Wolf SJ, Rizvi SM, Berthier CC, Hodgin JB, Beamer MA, Sarkar MK, Liang Y, Uppala R, Shao S, Zeng C, Harms PW, Verhaegen ME, Voorhees JJ, Wen F, Ward NL, Dlugosz AA, Kahlenberg JM, Gudjonsson JE
JCI insight 2019 Apr 18;4(8)
JCI insight 2019 Apr 18;4(8)
Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp.
Cheng HW, Onder L, Novkovic M, Soneson C, Lütge M, Pikor N, Scandella E, Robinson MD, Miyazaki JI, Tersteegen A, Sorg U, Pfeffer K, Rülicke T, Hehlgans T, Ludewig B
Nature communications 2019 Apr 15;10(1):1739
Nature communications 2019 Apr 15;10(1):1739
Human Adipose Tissue-Derived Mesenchymal Stem Cells Attenuate Atopic Dermatitis by Regulating the Expression of MIP-2, miR-122a-SOCS1 Axis, and Th1/Th2 Responses.
Kim M, Lee SH, Kim Y, Kwon Y, Park Y, Lee HK, Jung HS, Jeoung D
Frontiers in pharmacology 2018;9:1175
Frontiers in pharmacology 2018;9:1175
Inhaled TLR9 Agonist Renders Lung Tumors Permissive to PD-1 Blockade by Promoting Optimal CD4+ and CD8+ T-cell Interplay.
Gallotta M, Assi H, Degagné É, Kannan SK, Coffman RL, Guiducci C
Cancer research 2018 Sep 1;78(17):4943-4956
Cancer research 2018 Sep 1;78(17):4943-4956
Application of light sheet microscopy for qualitative and quantitative analysis of bronchus-associated lymphoid tissue in mice.
Mzinza DT, Fleige H, Laarmann K, Willenzon S, Ristenpart J, Spanier J, Sutter G, Kalinke U, Valentin-Weigand P, Förster R
Cellular & molecular immunology 2018 Oct;15(10):875-887
Cellular & molecular immunology 2018 Oct;15(10):875-887
Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection.
Alturaiki W, McFarlane AJ, Rose K, Corkhill R, McNamara PS, Schwarze J, Flanagan BF
Cytokine 2018 Oct;110:267-271
Cytokine 2018 Oct;110:267-271
Hyperactive gp130/STAT3-driven gastric tumourigenesis promotes submucosal tertiary lymphoid structure development.
Hill DG, Yu L, Gao H, Balic JJ, West A, Oshima H, McLeod L, Oshima M, Gallimore A, D'Costa K, Bhathal PS, Sievert W, Ferrero RL, Jenkins BJ, Jones GW
International journal of cancer 2018 Jul 1;143(1):167-178
International journal of cancer 2018 Jul 1;143(1):167-178
Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity.
Eddens T, Elsegeiny W, Garcia-Hernadez ML, Castillo P, Trevejo-Nunez G, Serody K, Campfield BT, Khader SA, Chen K, Rangel-Moreno J, Kolls JK
Cell reports 2017 Mar 28;18(13):3078-3090
Cell reports 2017 Mar 28;18(13):3078-3090
Ageing adversely affects the migration and function of marginal zone B cells.
Turner VM, Mabbott NA
Immunology 2017 Jul;151(3):349-362
Immunology 2017 Jul;151(3):349-362
Role of SIRPα in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen.
Respatika D, Saito Y, Washio K, Komori S, Kotani T, Okazawa H, Murata Y, Matozaki T
The Kobe journal of medical sciences 2017 Aug 10;63(1):E22-E29
The Kobe journal of medical sciences 2017 Aug 10;63(1):E22-E29
Brain inflammation is accompanied by peripheral inflammation in Cstb -/- mice, a model for progressive myoclonus epilepsy.
Okuneva O, Li Z, Körber I, Tegelberg S, Joensuu T, Tian L, Lehesjoki AE
Journal of neuroinflammation 2016 Nov 28;13(1):298
Journal of neuroinflammation 2016 Nov 28;13(1):298
Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia.
Lee KE, Spata M, Bayne LJ, Buza EL, Durham AC, Allman D, Vonderheide RH, Simon MC
Cancer discovery 2016 Mar;6(3):256-69
Cancer discovery 2016 Mar;6(3):256-69
Stromal cells as trend-setters for cells migrating into the lymph node.
Buettner M, Dittrich-Breiholz O, Falk CS, Lochner M, Smoczek A, Menzel F, Bornemann M, Bode U
Mucosal immunology 2015 May;8(3):640-9
Mucosal immunology 2015 May;8(3):640-9
Anti-CXCL13 antibody can inhibit the formation of gastric lymphoid follicles induced by Helicobacter infection.
Yamamoto K, Nishiumi S, Yang L, Klimatcheva E, Pandina T, Takahashi S, Matsui H, Nakamura M, Zauderer M, Yoshida M, Azuma T
Mucosal immunology 2014 Sep;7(5):1244-54
Mucosal immunology 2014 Sep;7(5):1244-54
CLEC-2 is required for development and maintenance of lymph nodes.
Bénézech C, Nayar S, Finney BA, Withers DR, Lowe K, Desanti GE, Marriott CL, Watson SP, Caamaño JH, Buckley CD, Barone F
Blood 2014 May 15;123(20):3200-7
Blood 2014 May 15;123(20):3200-7
Repetitive hypoxic preconditioning induces an immunosuppressed B cell phenotype during endogenous protection from stroke.
Monson NL, Ortega SB, Ireland SJ, Meeuwissen AJ, Chen D, Plautz EJ, Shubel E, Kong X, Li MK, Freriks LH, Stowe AM
Journal of neuroinflammation 2014 Jan 31;11:22
Journal of neuroinflammation 2014 Jan 31;11:22
IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs.
Fleige H, Ravens S, Moschovakis GL, Bölter J, Willenzon S, Sutter G, Häussler S, Kalinke U, Prinz I, Förster R
The Journal of experimental medicine 2014 Apr 7;211(4):643-51
The Journal of experimental medicine 2014 Apr 7;211(4):643-51
Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles.
Mionnet C, Mondor I, Jorquera A, Loosveld M, Maurizio J, Arcangeli ML, Ruddle NH, Nowak J, Aurrand-Lions M, Luche H, Bajénoff M
PLoS biology 2013 Oct;11(10):e1001672
PLoS biology 2013 Oct;11(10):e1001672
CXCL13 is elevated in Sjögren's syndrome in mice and humans and is implicated in disease pathogenesis.
Kramer JM, Klimatcheva E, Rothstein TL
Journal of leukocyte biology 2013 Nov;94(5):1079-89
Journal of leukocyte biology 2013 Nov;94(5):1079-89
IL-23 is required for long-term control of Mycobacterium tuberculosis and B cell follicle formation in the infected lung.
Khader SA, Guglani L, Rangel-Moreno J, Gopal R, Junecko BA, Fountain JJ, Martino C, Pearl JE, Tighe M, Lin YY, Slight S, Kolls JK, Reinhart TA, Randall TD, Cooper AM
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5402-7
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5402-7
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
Suzuki K, Maruya M, Kawamoto S, Sitnik K, Kitamura H, Agace WW, Fagarasan S
Immunity 2010 Jul 23;33(1):71-83
Immunity 2010 Jul 23;33(1):71-83
Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines.
St John AL, Abraham SN
Nature medicine 2009 Nov;15(11):1259-65
Nature medicine 2009 Nov;15(11):1259-65
Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice.
Gräbner R, Lötzer K, Döpping S, Hildner M, Radke D, Beer M, Spanbroek R, Lippert B, Reardon CA, Getz GS, Fu YX, Hehlgans T, Mebius RE, van der Wall M, Kruspe D, Englert C, Lovas A, Hu D, Randolph GJ, Weih F, Habenicht AJ
The Journal of experimental medicine 2009 Jan 16;206(1):233-48
The Journal of experimental medicine 2009 Jan 16;206(1):233-48
Role of lymphotoxin-alpha in cigarette smoke-induced inflammation and lymphoid neogenesis.
Demoor T, Bracke KR, Maes T, Vandooren B, Elewaut D, Pilette C, Joos GF, Brusselle GG
The European respiratory journal 2009 Aug;34(2):405-16
The European respiratory journal 2009 Aug;34(2):405-16
Suppression of established experimental autoimmune encephalomyelitis and formation of meningeal lymphoid follicles by lymphotoxin beta receptor-Ig fusion protein.
Columba-Cabezas S, Griguoli M, Rosicarelli B, Magliozzi R, Ria F, Serafini B, Aloisi F
Journal of neuroimmunology 2006 Oct;179(1-2):76-86
Journal of neuroimmunology 2006 Oct;179(1-2):76-86
Regulation of B1 cell migration by signals through Toll-like receptors.
Ha SA, Tsuji M, Suzuki K, Meek B, Yasuda N, Kaisho T, Fagarasan S
The Journal of experimental medicine 2006 Oct 30;203(11):2541-50
The Journal of experimental medicine 2006 Oct 30;203(11):2541-50
The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7296-302
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7296-302
The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7296-302
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7296-302
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL13/BLC/BCA-1 in Mouse Intestine. CXCL13/BLC/BCA-1 was detected in perfusion fixed frozen sections of mouse intestine (Peyer's patch) using Goat Anti-Mouse CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL13/BLC/BCA-1 and Neutralization by Mouse CXCL13/BLC/BCA-1 Antibody. Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin staining. Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA-1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL13/ BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470). The ND50 is typically 20-50 µg/mL.
