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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-29500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Afamin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, JC, BCL-1.
- Concentration
- 1 mg/mL
Submitted references Proteomics and multivariate modelling reveal sex-specific alterations in distinct regions of human carotid atheroma.
Ward LJ, Olausson P, Li W, Yuan XM
Biology of sex differences 2018 Dec 29;9(1):54
Biology of sex differences 2018 Dec 29;9(1):54
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Afamin Polyclonal Antibody (Product # PA5-29500). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2 . 7.5% SDS PAGE. Afamin Polyclonal Antibody (Product # PA5-29500) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Afamin Polyclonal Antibody (Product # PA5-29500). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2 . 7.5% SDS PAGE. Afamin Polyclonal Antibody (Product # PA5-29500) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Afamin Polyclonal Antibody (Product # PA5-29500). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1 . 7.5% SDS PAGE. Afamin Polyclonal Antibody (Product # PA5-29500) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AFM in methanol-fixed HeLa cells using an AFM polyclonal antibody (Product # PA5-29500) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Afamin was performed in paraffin-embedded human colon tissue using Afamin Polyclonal Antibody (Product # PA5-29500) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using AFM (Product # PA5-29500) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
