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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- GTX115443 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115443, RRID:AB_10719884
- Product name
- SCGN antibody [N2C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE GTX115443 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with SCGN antibody [N2C3] (GTX115443) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using SCGN(GTX115443) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SCGN antibody [N2C3] detects SCGN protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SCGN(GTX115443) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SCGN antibody [N2C3] detects SCGN Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green:SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
